Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_01065 and PS417_01070 overlap by 4 nucleotidesPS417_01070 and PS417_01075 are separated by 53 nucleotidesPS417_01075 and PS417_01080 overlap by 1 nucleotidesPS417_01080 and PS417_01085 are separated by 24 nucleotides PS417_01065: PS417_01065 - ABC transporter, at 233,194 to 234,309 _01065 PS417_01070: PS417_01070 - methionine ABC transporter substrate-binding protein, at 234,306 to 235,121 _01070 PS417_01075: PS417_01075 - N5,N10-methylene tetrahydromethanopterin reductase, at 235,175 to 236,527 _01075 PS417_01080: PS417_01080 - acyl-CoA dehydrogenase, at 236,527 to 237,720 _01080 PS417_01085: PS417_01085 - monooxygenase, at 237,745 to 238,986 _01085
Group Condition PS417_01065 PS417_01070 PS417_01075 PS417_01080 PS417_01085
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -0.4 -0.2 -1.5 -0.1
solid stress Fraxetin 3 mM; solid stress N.D. +0.0 -0.3 -1.1 +0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.4 -0.9 +0.3 -0.3
carbon source 2'-Deoxyinosine 5 mM (C) N.D. -0.0 -0.3 -1.0 -0.0
soil soil sample 5; outgrowth in LB N.D. -0.0 -0.7 +0.1 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.2 -0.2 -0.6 -0.4
rhizosphere rhizosphere sample 1; outgrowth in LB N.D. +0.3 -0.7 -0.6 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB N.D. -0.4 -0.4 -0.4 +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.2 +0.1 -0.6 -0.4
soil soil sample 4; outgrowth in LB N.D. +0.1 -0.9 +0.3 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.2 -0.8 +0.3 -0.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. +0.1 +0.3 -0.5 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.2 -0.4 -0.6 +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.4 -0.2 +0.4 -0.3
soil soil sample 6; outgrowth in LB N.D. -0.2 -0.6 +0.7 +0.0
carbon source octanoate (C) N.D. -0.1 -0.4 +0.6 -0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. +0.6 -0.3 -0.4 +0.1
carbon source L-Valine (C) N.D. -0.2 -0.4 +0.5 +0.3
carbon source Carbon source L-Citrulline 5 mM N.D. +0.3 +0.4 -0.6 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.0 -0.2 +0.9 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. -0.1 -0.3 +0.9 -0.2
phage P. simiae OR1 MOI 0.1 N.D. -0.3 -0.2 +0.4 +0.5
root root sample 6; outgrowth in LB N.D. -0.1 -0.3 +0.5 +0.5
soil soil sample 3; outgrowth in LB N.D. +0.3 -0.5 +0.7 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.0 -0.3 +0.7 +0.4
rhizosphere rhizosphere sample 2; outgrowth in LB N.D. -0.2 +0.4 +0.6 +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. +0.5 +0.1 +0.8 -0.2
carbon source L-Valine (C) N.D. +0.2 -0.1 +1.4 -0.3
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.2 -0.5 +1.6 +0.1
phage JP1 MOI 10 N.D. -0.0 +0.2 +0.7 +0.5
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