Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_01055 and PS417_01060 are separated by 423 nucleotidesPS417_01060 and PS417_01065 overlap by 11 nucleotidesPS417_01065 and PS417_01070 overlap by 4 nucleotidesPS417_01070 and PS417_01075 are separated by 53 nucleotides PS417_01055: PS417_01055 - methyltransferase, at 231,510 to 232,136 _01055 PS417_01060: PS417_01060 - ABC transporter permease, at 232,560 to 233,204 _01060 PS417_01065: PS417_01065 - ABC transporter, at 233,194 to 234,309 _01065 PS417_01070: PS417_01070 - methionine ABC transporter substrate-binding protein, at 234,306 to 235,121 _01070 PS417_01075: PS417_01075 - N5,N10-methylene tetrahydromethanopterin reductase, at 235,175 to 236,527 _01075
Group Condition PS417_01055 PS417_01060 PS417_01065 PS417_01070 PS417_01075
phage JP1 MOI 10 -0.6 -0.8 N.D. -0.2 -0.4
soil soil sample 4; outgrowth in LB -0.2 -0.4 N.D. +0.1 -0.9
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 -0.3 N.D. -0.0 -0.5
soil soil sample 5; outgrowth in LB +0.1 -0.3 N.D. -0.0 -0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.8 -0.3 N.D. +0.1 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.8 +0.2 N.D. -0.1 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 +0.3 N.D. -0.3 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 +0.1 N.D. -0.4 -0.9
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 -0.1 N.D. -0.2 -0.8
rhizosphere rhizosphere sample 1; outgrowth in LB -0.5 +0.3 N.D. +0.3 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 +0.3 N.D. +0.2 -0.4
soil soil sample 3; outgrowth in LB -0.1 -0.2 N.D. +0.3 -0.5
soil soil sample 6; outgrowth in LB +0.7 -0.1 N.D. -0.2 -0.6
phage P. simiae Grant Run MOI 10 -0.8 +0.3 N.D. +0.2 +0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.2 +0.2 N.D. -0.1 -0.6
root root sample 6; outgrowth in LB -0.4 +0.7 N.D. -0.1 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 -0.3 N.D. -0.3 +0.4
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.9 -0.7 N.D. +0.2 -0.5
carbon source 2-Deoxy-D-ribonic lithium salt 10 mM (C) -0.3 +0.3 N.D. -0.3 +0.2
seeds Growth on radish seeds for 72 hours -0.5 +0.3 N.D. +0.0 +0.3
solid stress Fraxetin 3 mM; solid stress -0.2 +0.8 N.D. +0.0 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 +0.7 N.D. -0.4 -0.2
phage P. simiae OR1 MOI 0.1 +0.8 +0.3 N.D. -0.3 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.6 -0.2 N.D. +0.6 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 +0.5 N.D. -0.1 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 +0.2 N.D. +0.5 -0.2
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 +0.5 N.D. +0.2 -0.2
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x +0.3 +0.5 N.D. +0.3 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.6 +0.3 N.D. +0.2 +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.6 +0.2 N.D. +0.4 +0.2
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