Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_10650 and PS417_10655 are separated by 298 nucleotidesPS417_10655 and PS417_10660 are separated by 1 nucleotidesPS417_10660 and PS417_10665 are separated by 134 nucleotidesPS417_10665 and PS417_10670 overlap by 4 nucleotides PS417_10650: PS417_10650 - methyltransferase, at 2,273,548 to 2,274,264 _10650 PS417_10655: PS417_10655 - LuxR family transcriptional regulator, at 2,274,563 to 2,275,189 _10655 PS417_10660: PS417_10660 - histidine kinase, at 2,275,191 to 2,278,805 _10660 PS417_10665: PS417_10665 - hypothetical protein, at 2,278,940 to 2,279,365 _10665 PS417_10670: PS417_10670 - 2,5-diketo-D-gluconic acid reductase, at 2,279,362 to 2,280,180 _10670
Group Condition PS417_10650 PS417_10655 PS417_10660 PS417_10665 PS417_10670
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -1.1 +0.0 -0.1 -1.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. -0.3 -0.2 -1.3 -0.2
phage P. simiae ORA MOI 1 N.D. -0.8 +0.1 -0.8 -0.4
seeds Growth on radish seeds for 72 hours N.D. -0.3 -0.1 -1.2 -0.3
agar plate interaction control Taped volatile agar plate with no fungus N.D. -0.5 +0.2 -1.7 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. -0.4 -0.1 -0.7 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -1.0 +0.0 -0.4 -0.3
carbon source Carbon source L-Ornithine 10 mM N.D. +0.2 -0.0 -1.7 -0.2
motility inner cut, LB soft agar motility assay N.D. -1.1 -0.2 -0.4 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. -0.6 -0.1 -0.8 +0.1
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -1.1 -0.1 -0.5 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. -1.3 +0.2 -0.6 +0.4
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.5 -0.1 +0.2 -0.8
soil soil sample 3; outgrowth in LB N.D. -0.6 +0.1 -0.7 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.3 +0.1 -0.9 +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.8 -0.0 -0.3 -1.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days N.D. +0.7 -0.2 -0.8 -0.1
phage P. simiae ORA MOI 0.1 N.D. -0.6 -0.0 +0.8 -0.4
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x N.D. +0.5 +0.1 -0.9 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.6 +0.0 +0.9 -0.2
carbon source 2'-Deoxyinosine 5 mM (C) N.D. -0.6 -0.1 +0.9 +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. -0.1 +0.0 -0.5 +1.1
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. +1.0 -0.0 -0.7 +0.3
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. +0.9 +0.0 -0.6 +0.3
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. -0.0 +0.1 +1.0 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.7 +0.0 +0.2 +1.2
phage Wcs_1 N.D. +0.5 +0.0 +0.9 -0.3
carbon source L-Valine (C) N.D. +0.9 +0.3 +0.4 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +1.5 -0.0 +0.3 +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.3 -0.2 +2.0 -0.1
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