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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_10570 and PS417_10575 are separated by 148 nucleotides
PS417_10575 and PS417_10580 are separated by 18 nucleotides
PS417_10580 and PS417_10585 are separated by 11 nucleotides
PS417_10585 and PS417_10590 are separated by 153 nucleotides
PS417_10570: PS417_10570 - cysteine protease, at 2,256,380 to 2,258,239
_10570
PS417_10575: PS417_10575 - DNA alkylation repair protein, at 2,258,388 to 2,259,488
_10575
PS417_10580: PS417_10580 - glutamine synthetase, at 2,259,507 to 2,260,853
_10580
PS417_10585: PS417_10585 - Cro/Cl family transcriptional regulator, at 2,260,865 to 2,261,422
_10585
PS417_10590: PS417_10590 - hypothetical protein, at 2,261,576 to 2,261,884
_10590
Group
Condition
PS417
_10570
PS417
_10575
PS417
_10580
PS417
_10585
PS417
_10590
root
root sample 6; outgrowth in LB
-0.1
-0.8
-0.6
-0.5
N.D.
phage
P. simiae Grant Run MOI 0.1
-0.1
-1.3
-0.2
-0.1
N.D.
stress
methylglyoxal 0.032 vol%
-0.1
-0.9
-0.4
-0.3
N.D.
soil
soil sample 5; outgrowth in LB
-0.1
-0.7
-0.6
-0.2
N.D.
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.2
-1.1
-0.3
-0.1
N.D.
phage
Wcs_1
-0.1
-1.4
+0.2
-0.2
N.D.
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.4
-1.0
+0.2
-0.3
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.0
+0.1
-1.4
-0.1
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.3
-0.9
-0.3
-0.3
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.1
-0.9
-0.2
+0.5
N.D.
stress
Phosphomycin 0.001 mg/ml
-0.1
-0.7
+0.4
-0.3
N.D.
motility
outer cut, LB soft agar motility assay
-0.1
-1.0
+0.4
+0.0
N.D.
carbon source
L-Valine (C)
-0.4
-0.6
+0.4
-0.2
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.1
+0.5
-0.6
-0.3
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
+0.1
-1.0
+0.1
N.D.
seeds
Growth on radish seeds for 72 hours
+0.1
-0.8
+0.2
+0.3
N.D.
phage
P. simiae OR Antrim MOI 0.1
+0.2
+0.3
-0.7
+0.3
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
+0.7
-0.6
+0.1
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.1
+1.3
-1.0
+0.0
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.0
+1.0
-0.3
-0.1
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
+1.4
+0.3
-0.7
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
+0.9
-0.4
+0.6
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
+0.1
-0.6
+0.3
+1.2
N.D.
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.1
+1.1
-0.4
+0.3
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
+0.7
-0.2
+0.3
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
+0.8
-0.2
+0.5
N.D.
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
+0.1
+1.1
+0.2
+0.3
N.D.
supernatant
RCH2_defined_glucose with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
-0.0
+1.0
+0.3
+0.4
N.D.
phage
JP1 MOI 0.1
-0.0
+1.0
+0.5
+0.2
N.D.
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
+0.0
-0.0
+1.7
+0.1
N.D.
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