Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_10520 and PS417_10525 are separated by 34 nucleotidesPS417_10525 and PS417_10530 are separated by 133 nucleotidesPS417_10530 and PS417_10535 are separated by 59 nucleotidesPS417_10535 and PS417_10540 are separated by 546 nucleotides PS417_10520: PS417_10520 - succinyl-CoA:3-ketoacid-CoA transferase, at 2,245,036 to 2,245,695 _10520 PS417_10525: PS417_10525 - succinyl-CoA:3-ketoacid-CoA transferase, at 2,245,730 to 2,246,428 _10525 PS417_10530: PS417_10530 - LysR family transcriptional regulator, at 2,246,562 to 2,247,467 _10530 PS417_10535: PS417_10535 - hypothetical protein, at 2,247,527 to 2,247,856 _10535 PS417_10540: PS417_10540 - NAD-dependent deacetylase, at 2,248,403 to 2,249,248 _10540
Group Condition PS417_10520 PS417_10525 PS417_10530 PS417_10535 PS417_10540
carbon source L-Valine (C) -1.0 N.D. +0.1 -3.9 -1.0
carbon source 2-Deoxy-D-ribonic lithium salt 10 mM (C) -2.7 N.D. -1.8 -0.2 -0.5
carbon source L-Valine (C) +0.0 N.D. -0.3 -3.3 -0.7
carbon source 2-Deoxy-D-ribonic lithium salt 10 mM (C) -2.5 N.D. -1.5 -0.2 -0.0
carbon source 2-Deoxy-D-ribonic lithium salt (C) -2.3 N.D. -1.2 -0.1 -0.1
carbon source 2'-Deoxyinosine 5 mM (C) -0.9 N.D. -0.2 -1.0 -0.2
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x -1.7 N.D. -0.5 +0.1 -0.3
carbon source L-Leucine (C) -1.0 N.D. -0.2 -0.8 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 N.D. -0.6 -0.4 -0.9
carbon source 2-Deoxy-D-Ribose 10 mM (C) -1.3 N.D. -0.7 -0.1 -0.1
carbon source 2-Deoxy-D-Ribose 10 mM (C) -1.0 N.D. -0.5 -0.2 -0.4
carbon source L-Leucine (C) -1.0 N.D. -0.4 -0.8 +0.1
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x -1.2 N.D. -0.5 +0.3 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.6 N.D. -0.5 -0.5 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.1 N.D. -0.0 -1.3 +0.6
carbon source 2'-Deoxyinosine 5 mM (C) -1.6 N.D. +0.3 +0.1 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -1.0 N.D. +0.2 -0.6 -0.1
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x -1.6 N.D. -0.4 +0.2 +0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.6 N.D. +0.4 -0.2 -0.9
phage P. simiae Grant Run MOI 0.1 -0.4 N.D. -0.4 -0.6 +0.5
phage P. simiae ORA MOI 0.1 -0.1 N.D. +0.1 -1.4 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.7 N.D. +0.1 -0.7 +0.4
agar plate interaction control Taped volatile agar plate with no fungus -0.5 N.D. -0.2 -0.6 +0.5
rhizosphere rhizosphere sample 8; outgrowth in LB -0.6 N.D. +0.3 -0.8 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -1.1 N.D. -0.0 +0.6 -0.2
soil soil sample 6; outgrowth in LB -1.3 N.D. +0.2 +0.5 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.9 N.D. +0.1 -0.2 +0.7
stress methylglyoxal 0.032 vol% +0.1 N.D. +0.3 -1.0 +0.5
carbon source Putrescine (C) +0.4 N.D. +0.2 -1.1 +0.4
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.1 N.D. +0.6 +0.6 +0.5
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