Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_10490 and PS417_10495 are separated by 54 nucleotidesPS417_10495 and PS417_10500 are separated by 147 nucleotidesPS417_10500 and PS417_10505 are separated by 0 nucleotidesPS417_10505 and PS417_10510 are separated by 149 nucleotides PS417_10490: PS417_10490 - delta-aminolevulinic acid dehydratase, at 2,239,364 to 2,240,338 _10490 PS417_10495: PS417_10495 - lysine transporter LysE, at 2,240,393 to 2,241,007 _10495 PS417_10500: PS417_10500 - lipoprotein, at 2,241,155 to 2,242,240 _10500 PS417_10505: PS417_10505 - hypothetical protein, at 2,242,241 to 2,243,089 _10505 PS417_10510: PS417_10510 - esterase, at 2,243,239 to 2,243,655 _10510
Group Condition PS417_10490 PS417_10495 PS417_10500 PS417_10505 PS417_10510
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X -0.9 -0.1 -0.3 -0.3 N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -1.1 -0.3 +0.0 -0.2 N.D.
carbon source L-Valine (C) -0.6 -0.3 +0.0 -0.5 N.D.
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.5 -0.0 -0.3 -0.6 N.D.
carbon source L-Leucine (C) -0.8 -0.1 +0.1 -0.3 N.D.
agar plate interaction Taped volatile agar plate with Trichoderma atroviridae IMI -0.9 -0.4 +0.0 +0.2 N.D.
phage P. simiae Grant Run MOI 10 +0.2 -0.6 -0.3 -0.4 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.1 -0.3 +0.2 -0.7 N.D.
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.6 -0.1 +0.4 -0.6 N.D.
no stress control on plate growth on LB_noSalt plate -0.5 +0.2 +0.2 -0.7 N.D.
phage P. simiae Grant Run MOI 0.1 -0.9 +0.0 -0.3 +0.5 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.7 +0.3 -0.1 +0.1 N.D.
phage P. simiae OR Antrim MOI 0.1 +0.1 -0.4 -0.3 +0.4 N.D.
solid stress Fraxetin 3 mM; solid stress +0.7 +0.1 -0.1 -0.8 N.D.
phage P. simiae OR Antrim MOI 1 -0.4 -0.1 -0.2 +0.6 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.5 +0.0 +0.2 -0.6 N.D.
seeds Growth on radish seeds for 72 hours +0.3 +0.1 +0.4 -0.7 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 +0.4 +0.3 -0.6 N.D.
phage Wcs_0.1 +0.8 -0.2 -0.1 +0.1 N.D.
solid stress Fraxetin 1 mM; solid stress +0.6 +0.5 -0.2 -0.1 N.D.
phage JP1 MOI 10 +1.1 -0.5 -0.1 +0.3 N.D.
seeds Growth on radish seeds for 72 hours +1.3 -0.1 -0.1 -0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.7 +0.4 +0.0 -0.2 N.D.
phage JP1 MOI 0.1 +0.8 -0.4 -0.1 +0.6 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.3 -0.1 +0.0 +0.1 N.D.
phage P. simiae OR1 MOI 0.1 +1.2 -0.2 +0.1 +0.2 N.D.
root root sample 6; outgrowth in LB +0.5 +0.3 +0.1 +0.5 N.D.
soil soil sample 5; outgrowth in LB +0.9 +0.2 -0.0 +0.4 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.8 -0.0 +0.1 +0.9 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +2.0 +0.2 +0.1 +0.0 N.D.
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