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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_10480 and PS417_10485 are separated by 3 nucleotides
PS417_10485 and PS417_10490 are separated by 116 nucleotides
PS417_10490 and PS417_10495 are separated by 54 nucleotides
PS417_10495 and PS417_10500 are separated by 147 nucleotides
PS417_10480: PS417_10480 - glutathione S-transferase, at 2,237,703 to 2,238,401
_10480
PS417_10485: PS417_10485 - phenazine biosynthesis protein PhzF, at 2,238,405 to 2,239,247
_10485
PS417_10490: PS417_10490 - delta-aminolevulinic acid dehydratase, at 2,239,364 to 2,240,338
_10490
PS417_10495: PS417_10495 - lysine transporter LysE, at 2,240,393 to 2,241,007
_10495
PS417_10500: PS417_10500 - lipoprotein, at 2,241,155 to 2,242,240
_10500
Group
Condition
PS417
_10480
PS417
_10485
PS417
_10490
PS417
_10495
PS417
_10500
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-2.2
-0.3
+0.0
-0.1
-0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-1.7
-0.0
-0.2
-0.2
-0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.6
-0.1
+0.1
-0.0
-0.0
carbon source
L-Valine (C)
-1.0
-0.4
-0.7
+0.2
+0.2
stress
Dimethyl Sulfoxide 5 vol%
-1.2
-0.1
-0.7
+0.2
+0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.9
+0.1
+0.0
+0.0
+0.0
carbon source
L-Leucine (C)
-0.7
-0.2
-0.8
-0.1
+0.1
no phage control
Time6-nophage
-1.0
+0.0
-0.6
-0.3
+0.2
phage moi 100
Time6-phageFRS
-1.2
-0.0
-0.5
+0.1
+0.1
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-1.4
-0.5
+0.1
+0.4
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.6
+0.1
-0.7
+0.3
-0.1
solid stress
80% Methanol 1 mM; solid stress
-1.1
-0.1
+0.5
-0.0
-0.1
supernatant control
Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
-1.0
+0.3
+0.1
-0.3
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.1
+0.3
-0.0
+0.3
+0.2
stress
Doxycycline hyclate 0.001 mg/ml
-0.7
-0.6
+0.8
+0.1
+0.0
phage
P. simiae Grant Run MOI 10
-0.3
+0.6
+0.2
-0.6
-0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.9
-0.2
+0.7
+0.4
+0.0
phage
JP1 MOI 10
+0.3
-0.6
+1.1
-0.5
-0.1
carbon source
L-Valine (C)
+1.4
-0.1
-0.6
-0.3
+0.0
phage
JP1 MOI 0.1
-0.3
+0.3
+0.8
-0.4
-0.1
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
+1.6
+0.4
-1.1
-0.3
+0.0
phage
Wcs_0.1
+0.5
-0.2
+0.8
-0.2
-0.1
phage
P. simiae OR1 MOI 0.1
+0.2
-0.5
+1.2
-0.2
+0.1
seeds
Growth on radish seeds for 72 hours
+0.7
-0.3
+0.3
+0.1
+0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.8
-0.1
+2.0
+0.2
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+1.7
-0.1
+0.3
-0.3
-0.2
seeds
Growth on radish seeds for 72 hours
+0.1
+0.3
+1.3
-0.1
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+1.5
-0.4
+0.4
-0.0
+0.3
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+2.6
-0.2
+0.1
-0.3
+0.0
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+3.6
-0.0
+0.3
+0.1
-0.1
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