Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_10470 and PS417_10475 are separated by 44 nucleotidesPS417_10475 and PS417_10480 are separated by 250 nucleotidesPS417_10480 and PS417_10485 are separated by 3 nucleotidesPS417_10485 and PS417_10490 are separated by 116 nucleotides PS417_10470: PS417_10470 - membrane protein, at 2,235,651 to 2,236,706 _10470 PS417_10475: PS417_10475 - GTP-binding protein, at 2,236,751 to 2,237,452 _10475 PS417_10480: PS417_10480 - glutathione S-transferase, at 2,237,703 to 2,238,401 _10480 PS417_10485: PS417_10485 - phenazine biosynthesis protein PhzF, at 2,238,405 to 2,239,247 _10485 PS417_10490: PS417_10490 - delta-aminolevulinic acid dehydratase, at 2,239,364 to 2,240,338 _10490
Group Condition PS417_10470 PS417_10475 PS417_10480 PS417_10485 PS417_10490
carbon source L-Valine (C) -0.3 -0.1 -1.0 -0.4 -0.7
stress Dimethyl Sulfoxide 5 vol% -0.2 +0.1 -1.2 -0.1 -0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 -0.0 -1.9 +0.1 +0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.2 +0.2 -1.7 -0.0 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 -0.2 -1.4 -0.5 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.6 +0.1 -2.2 -0.3 +0.0
supernatant control Vogels_fungal_media 0.1X -0.4 -0.5 -0.8 -0.3 +0.3
no phage control Time6-nophage +0.2 +0.2 -1.0 +0.0 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.6 +0.0 -1.6 -0.1 +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.6 -0.1 -1.1 -0.0 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 +0.4 -1.0 -0.0 -0.4
stress Doxycycline hyclate 0.001 mg/ml +0.0 +0.1 -0.7 -0.6 +0.8
soil soil sample 7; outgrowth in LB +0.1 +0.6 -1.1 +0.4 +0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 +0.4 -0.6 -0.2 +0.8
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 -0.3 -0.2 +0.5 +0.8
carbon source L-Valine (C) -0.1 -0.1 +1.4 -0.1 -0.6
soil soil sample 5; outgrowth in LB -0.6 +0.3 -0.3 +0.2 +0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.3 -0.1 -0.8 -0.1 +2.0
carbon source 2'-Deoxyinosine 5 mM (C) +0.6 -0.1 +0.8 -0.5 +0.3
phage JP1 MOI 10 +0.3 +0.1 +0.3 -0.6 +1.1
seeds Growth on radish seeds for 72 hours -0.1 -0.3 +0.1 +0.3 +1.3
phage P. simiae OR1 MOI 0.1 +0.3 +0.1 +0.2 -0.5 +1.2
soil soil sample 6; outgrowth in LB -0.3 +0.4 +0.2 +0.7 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.6 -0.2 +1.1 +0.2 -0.2
phage P. simiae Grant Run MOI 0.1 +0.4 +0.1 +1.4 -0.1 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 -0.2 +1.5 -0.4 +0.4
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.2 +0.6 +1.6 +0.4 -1.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.2 +0.1 +2.6 -0.2 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.8 -0.2 +1.7 -0.1 +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.1 -0.4 +3.6 -0.0 +0.3
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