Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_10420 and PS417_10425 are separated by 57 nucleotidesPS417_10425 and PS417_10430 are separated by 115 nucleotidesPS417_10430 and PS417_10435 are separated by 69 nucleotidesPS417_10435 and PS417_10440 are separated by 226 nucleotides PS417_10420: PS417_10420 - shikimate dehydrogenase, at 2,226,422 to 2,227,240 _10420 PS417_10425: PS417_10425 - hypothetical protein, at 2,227,298 to 2,227,945 _10425 PS417_10430: PS417_10430 - hypothetical protein, at 2,228,061 to 2,228,570 _10430 PS417_10435: PS417_10435 - serine/threonine protein kinase, at 2,228,640 to 2,229,860 _10435 PS417_10440: PS417_10440 - MFS transporter, at 2,230,087 to 2,231,244 _10440
Group Condition PS417_10420 PS417_10425 PS417_10430 PS417_10435 PS417_10440
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.9 -0.2 N.D. -0.9 -0.0
phage P. simiae ORA MOI 0.1 -0.3 -0.9 N.D. -0.3 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.2 -0.4 N.D. +0.2 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.3 -1.5 N.D. -0.7 +0.0
root root sample 6; outgrowth in LB -0.1 -1.5 N.D. -0.0 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.0 -1.4 N.D. -0.5 +0.2
phage P. simiae OR Antrim MOI 1 -0.3 -1.0 N.D. -0.5 +0.2
motility inner cut, LB soft agar motility assay -0.2 -1.1 N.D. +0.1 -0.4
soil soil sample 7; outgrowth in LB -0.1 -1.1 N.D. +0.4 -0.6
stress methylglyoxal 0.032 vol% +0.5 -1.9 N.D. +0.0 -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 -0.2 N.D. -1.0 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 -1.4 N.D. -0.0 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 -0.6 N.D. -0.4 -0.6
carbon source Carbon source D-Mannitol 10 mM -0.3 -1.0 N.D. +0.5 -0.3
phage Wcs_0.1 +0.2 -1.4 N.D. +0.3 +0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 -1.1 N.D. +0.1 +0.6
exudates RCH2_defined_glucose with Brachypodium distachyon 13-day exudates 1x +0.2 -1.0 N.D. -0.3 +0.5
carbon source 2'-Deoxyinosine 5 mM (C) +0.2 +0.6 N.D. -0.9 -0.3
solid stress Fraxetin 3 mM; solid stress +0.2 +0.7 N.D. -0.6 -0.4
seeds Growth on radish seeds for 72 hours -0.5 +0.9 N.D. -0.5 -0.0
phage P. simiae Grant Run MOI 0.1 -0.5 +0.8 N.D. -0.4 +0.1
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x +0.2 -0.8 N.D. +0.3 +0.5
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.4 +0.7 N.D. -0.5 -0.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.2 +0.9 N.D. +0.3 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 +1.0 N.D. +0.3 -0.4
seeds Growth on radish seeds for 72 hours -0.5 +1.4 N.D. +0.7 -0.8
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.1 +1.5 N.D. +0.1 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.2 +1.7 N.D. +0.1 -0.3
phage P. simiae Grant Run MOI 0.1 +0.1 +1.1 N.D. -0.3 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 +0.5 N.D. +0.7 +0.4
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