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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_10410 and PS417_10415 are separated by 225 nucleotides
PS417_10415 and PS417_10420 are separated by 156 nucleotides
PS417_10420 and PS417_10425 are separated by 57 nucleotides
PS417_10425 and PS417_10430 are separated by 115 nucleotides
PS417_10410: PS417_10410 - hypothetical protein, at 2,225,471 to 2,225,665
_10410
PS417_10415: PS417_10415 - hypothetical protein, at 2,225,891 to 2,226,265
_10415
PS417_10420: PS417_10420 - shikimate dehydrogenase, at 2,226,422 to 2,227,240
_10420
PS417_10425: PS417_10425 - hypothetical protein, at 2,227,298 to 2,227,945
_10425
PS417_10430: PS417_10430 - hypothetical protein, at 2,228,061 to 2,228,570
_10430
Group
Condition
PS417
_10410
PS417
_10415
PS417
_10420
PS417
_10425
PS417
_10430
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.7
-1.2
-0.4
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
N.D.
-0.5
-0.0
-1.4
N.D.
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
-0.4
-0.2
-1.2
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.8
-0.0
-0.8
N.D.
phage
P. simiae ORA MOI 0.1
N.D.
-0.4
-0.3
-0.9
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.0
-0.1
-1.4
N.D.
soil
soil sample 7; outgrowth in LB
N.D.
-0.4
-0.1
-1.1
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
-0.1
-0.2
-1.1
N.D.
liquid stress
Scopoletin 3 mM; liquid stress
N.D.
-0.2
-0.0
-1.2
N.D.
nophagecontrol
TIme6-nophage
N.D.
-0.2
-0.2
-1.0
N.D.
root
root sample 6; outgrowth in LB
N.D.
+0.2
-0.1
-1.5
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
N.D.
-0.2
-0.1
-1.1
N.D.
phage
Wcs_0.1
N.D.
-0.1
+0.2
-1.4
N.D.
stress
Doxycycline hyclate 0.001 mg/ml
N.D.
-0.0
+0.1
-1.4
N.D.
stress
methylglyoxal 0.032 vol%
N.D.
+0.1
+0.5
-1.9
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
N.D.
-0.0
+0.3
-1.5
N.D.
carbon source
Carbon source L-Citrulline 10 mM
N.D.
+0.1
+0.1
-1.3
N.D.
carbon source
Carbon source D-Mannitol 10 mM
N.D.
+0.1
-0.3
-1.0
N.D.
motility
inner cut, LB soft agar motility assay
N.D.
+0.2
-0.2
-1.1
N.D.
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
-0.1
+0.2
-1.2
N.D.
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB
N.D.
+0.1
+0.1
-1.2
N.D.
exudates
RCH2_defined_glucose with Brachypodium distachyon 13-day exudates 1x
N.D.
-0.1
+0.2
-1.0
N.D.
carbon source
Gly-Glu (C)
N.D.
+0.3
+0.2
-1.4
N.D.
supernatant
Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
N.D.
-0.3
+0.4
-0.7
N.D.
phage
P. simiae Grant Run MOI 0.1
N.D.
-0.2
-0.5
+0.8
N.D.
seeds
Growth on radish seeds for 72 hours
N.D.
-0.1
-0.5
+0.9
N.D.
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
N.D.
-0.3
-0.2
+0.9
N.D.
seeds
Growth on radish seeds for 72 hours
N.D.
+0.1
-0.5
+1.4
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
N.D.
-0.1
-0.2
+1.7
N.D.
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
N.D.
+0.1
-0.1
+1.5
N.D.
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