Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_10410 and PS417_10415 are separated by 225 nucleotidesPS417_10415 and PS417_10420 are separated by 156 nucleotidesPS417_10420 and PS417_10425 are separated by 57 nucleotidesPS417_10425 and PS417_10430 are separated by 115 nucleotides PS417_10410: PS417_10410 - hypothetical protein, at 2,225,471 to 2,225,665 _10410 PS417_10415: PS417_10415 - hypothetical protein, at 2,225,891 to 2,226,265 _10415 PS417_10420: PS417_10420 - shikimate dehydrogenase, at 2,226,422 to 2,227,240 _10420 PS417_10425: PS417_10425 - hypothetical protein, at 2,227,298 to 2,227,945 _10425 PS417_10430: PS417_10430 - hypothetical protein, at 2,228,061 to 2,228,570 _10430
Group Condition PS417_10410 PS417_10415 PS417_10420 PS417_10425 PS417_10430
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.7 -1.2 -0.4 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. -0.5 -0.0 -1.4 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.4 -0.2 -1.2 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.8 -0.0 -0.8 N.D.
phage P. simiae ORA MOI 0.1 N.D. -0.4 -0.3 -0.9 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.0 -0.1 -1.4 N.D.
soil soil sample 7; outgrowth in LB N.D. -0.4 -0.1 -1.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.1 -0.2 -1.1 N.D.
liquid stress Scopoletin 3 mM; liquid stress N.D. -0.2 -0.0 -1.2 N.D.
nophagecontrol TIme6-nophage N.D. -0.2 -0.2 -1.0 N.D.
root root sample 6; outgrowth in LB N.D. +0.2 -0.1 -1.5 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. -0.2 -0.1 -1.1 N.D.
phage Wcs_0.1 N.D. -0.1 +0.2 -1.4 N.D.
stress Doxycycline hyclate 0.001 mg/ml N.D. -0.0 +0.1 -1.4 N.D.
stress methylglyoxal 0.032 vol% N.D. +0.1 +0.5 -1.9 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -0.0 +0.3 -1.5 N.D.
carbon source Carbon source L-Citrulline 10 mM N.D. +0.1 +0.1 -1.3 N.D.
carbon source Carbon source D-Mannitol 10 mM N.D. +0.1 -0.3 -1.0 N.D.
motility inner cut, LB soft agar motility assay N.D. +0.2 -0.2 -1.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.1 +0.2 -1.2 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB N.D. +0.1 +0.1 -1.2 N.D.
exudates RCH2_defined_glucose with Brachypodium distachyon 13-day exudates 1x N.D. -0.1 +0.2 -1.0 N.D.
carbon source Gly-Glu (C) N.D. +0.3 +0.2 -1.4 N.D.
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x N.D. -0.3 +0.4 -0.7 N.D.
phage P. simiae Grant Run MOI 0.1 N.D. -0.2 -0.5 +0.8 N.D.
seeds Growth on radish seeds for 72 hours N.D. -0.1 -0.5 +0.9 N.D.
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. -0.3 -0.2 +0.9 N.D.
seeds Growth on radish seeds for 72 hours N.D. +0.1 -0.5 +1.4 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. -0.1 -0.2 +1.7 N.D.
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.1 -0.1 +1.5 N.D.
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