Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_10345 and PS417_10350 are separated by 173 nucleotidesPS417_10350 and PS417_10355 are separated by 7 nucleotidesPS417_10355 and PS417_10360 are separated by 90 nucleotidesPS417_10360 and PS417_10365 are separated by 105 nucleotides PS417_10345: PS417_10345 - MFS transporter, at 2,210,850 to 2,212,076 _10345 PS417_10350: PS417_10350 - multidrug MFS transporter, at 2,212,250 to 2,213,542 _10350 PS417_10355: PS417_10355 - transcriptional regulator, at 2,213,550 to 2,216,420 _10355 PS417_10360: PS417_10360 - MFS transporter, at 2,216,511 to 2,217,725 _10360 PS417_10365: PS417_10365 - AraC family transcriptional regulator, at 2,217,831 to 2,218,646 _10365
Group Condition PS417_10345 PS417_10350 PS417_10355 PS417_10360 PS417_10365
rhizosphere rhizosphere sample 1; outgrowth in LB -0.3 -0.6 -0.1 -0.6 -0.2
agar plate interaction control Taped volatile agar plate with no fungus -0.5 -0.2 -0.0 -1.0 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 -0.5 -0.2 -0.3 -0.6
carbon source L-Valine (C) +0.1 -0.5 -0.4 -0.6 -0.1
seeds Growth on radish seeds for 72 hours -0.3 +0.1 -0.3 -0.4 -0.6
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.8 -0.0 +0.0 -0.3 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.7 +0.0 -0.1 -0.3 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 +0.3 -0.4 -0.6 -0.5
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 +0.3 -0.1 -0.8 -0.0
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.7 -0.0 +0.1 -0.5 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 -0.2 -0.2 -0.4 -0.5
stress Gentamicin 0.008 mg/ml -1.1 -0.0 -0.0 +0.3 -0.1
phage Wcs_1 -0.7 -0.1 +0.3 -0.3 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.8X; growth supplemented with 0.4X LB +0.2 +0.2 -0.4 -0.3 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.9 -0.4 +0.3 -0.2 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.4 +0.1 +0.3 -0.1 -0.6
soil soil sample 7; outgrowth in LB +0.6 -0.4 +0.0 +0.1 -0.8
seeds Growth on radish seeds for 72 hours -0.6 -0.3 +0.1 +0.3 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 +0.5 -0.3 -0.1 -0.2
carbon source 2'-Deoxyinosine 5 mM (C) +0.3 -0.3 -0.2 +0.3 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 +0.3 -0.3 -0.1 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 -0.2 -0.3 -0.3 +0.4
phage P. simiae OR Antrim MOI 1 +0.7 -0.3 +0.1 +0.0 -0.6
phage P. simiae Grant Run MOI 0.1 +0.7 +0.2 +0.1 -0.2 -0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 +0.4 +0.1 -0.4 -0.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.4 +0.2 -0.2 -0.4 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.0 -0.2 -0.3 +0.4 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 +0.5 -0.3 -0.4 +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 +0.2 -0.0 -0.4 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.0 +0.7 +0.3 +0.1 -0.4
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