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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_10330 and PS417_10335 are separated by 198 nucleotides
PS417_10335 and PS417_10340 are separated by 147 nucleotides
PS417_10340 and PS417_10345 overlap by 20 nucleotides
PS417_10345 and PS417_10350 are separated by 173 nucleotides
PS417_10330: PS417_10330 - histidine kinase, at 2,207,573 to 2,209,135
_10330
PS417_10335: PS417_10335 - peroxiredoxin, at 2,209,334 to 2,209,750
_10335
PS417_10340: PS417_10340 - alcohol dehydrogenase, at 2,209,898 to 2,210,869
_10340
PS417_10345: PS417_10345 - MFS transporter, at 2,210,850 to 2,212,076
_10345
PS417_10350: PS417_10350 - multidrug MFS transporter, at 2,212,250 to 2,213,542
_10350
Group
Condition
PS417
_10330
PS417
_10335
PS417
_10340
PS417
_10345
PS417
_10350
solid stress
Fraxetin 3 mM; solid stress
-1.7
-1.8
+0.2
-0.1
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.9
-1.4
-0.8
+0.1
+0.2
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.5
-1.9
-0.0
+0.1
-0.5
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.6
-0.6
-0.7
-0.2
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.0
-1.2
-1.3
-0.1
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.6
-1.0
-0.5
+0.0
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.7
-1.0
-0.4
-0.0
-0.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.7
-0.3
-0.6
-0.4
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.7
-0.9
-0.5
+0.2
-0.2
soil
soil sample 4; outgrowth in LB
+0.3
-1.6
-0.0
-0.5
-0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Cysteine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.3
-1.7
-0.3
+0.2
+0.2
nophagecontrol
Only library
-0.9
-0.7
-0.7
+0.7
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.4
-1.6
+0.4
+0.1
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.5
+0.4
-0.8
+0.3
+0.0
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.9
-0.4
-1.1
+0.6
+0.2
carbon source
2'-Deoxyinosine 5 mM (C)
+0.6
-1.2
-0.5
-0.4
+0.0
no phage control
Time6-nophage
-0.9
-1.0
+0.3
+0.2
-0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
-0.6
-0.9
-0.5
+0.3
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.2
+0.4
-1.0
-0.2
-0.4
phage
JP1 MOI 10
-0.3
-1.3
+0.3
+0.1
-0.0
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-1.0
-0.1
-0.5
-0.0
+0.7
agar plate interaction control
Taped volatile agar plate with no fungus
+0.8
-1.0
+0.3
-0.5
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
-0.6
-0.5
+0.4
-0.2
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.8
+0.6
-1.0
+0.5
+0.4
seeds
Growth on radish seeds for 72 hours
-0.4
+1.0
+0.1
-0.6
-0.3
liquid stress
Fraxetin 2 mM; liquid stress
-1.0
+0.4
+0.4
+0.3
-0.1
phage
P. simiae Grant Run MOI 0.1
-0.8
+0.2
-0.3
+0.7
+0.2
in planta
Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.0
+0.7
+0.8
-0.4
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
-0.4
+1.0
-0.3
+0.5
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+1.6
+0.9
+0.5
+0.2
+0.6
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