Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_10305 and PS417_10310 are separated by 42 nucleotidesPS417_10310 and PS417_10315 are separated by 31 nucleotidesPS417_10315 and PS417_10320 are separated by 1 nucleotidesPS417_10320 and PS417_10325 are separated by 84 nucleotides PS417_10305: PS417_10305 - hypothetical protein, at 2,204,737 to 2,205,000 _10305 PS417_10310: PS417_10310 - hypothetical protein, at 2,205,043 to 2,205,255 _10310 PS417_10315: PS417_10315 - cytochrome C biogenesis protein, at 2,205,287 to 2,206,495 _10315 PS417_10320: PS417_10320 - hypothetical protein, at 2,206,497 to 2,207,117 _10320 PS417_10325: PS417_10325 - chemotaxis protein CheY, at 2,207,202 to 2,207,573 _10325
Group Condition PS417_10305 PS417_10310 PS417_10315 PS417_10320 PS417_10325
soil soil sample 6; outgrowth in LB -2.4 N.D. -0.3 +0.3 N.D.
seeds Growth on radish seeds for 72 hours -0.6 N.D. -0.5 -1.2 N.D.
root root sample 6; outgrowth in LB -1.2 N.D. -0.1 -0.7 N.D.
carbon source 2'-Deoxyinosine 5 mM (C) -1.3 N.D. -0.3 -0.3 N.D.
phage moi 100 Time6-phageFRS -1.7 N.D. +0.2 -0.3 N.D.
phage JP1 MOI 1 -1.0 N.D. +0.3 -1.0 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -1.7 N.D. -0.1 +0.1 N.D.
nophagecontrol Only library -0.7 N.D. -0.2 -0.8 N.D.
phage JP1 MOI 1 -0.3 N.D. +0.0 -1.4 N.D.
carbon source Carbon source D-Mannitol 5 mM -0.8 N.D. -0.2 -0.8 N.D.
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x -1.1 N.D. +0.1 -0.6 N.D.
stress Bacitracin 2 mg/ml -1.0 N.D. -0.1 -0.5 N.D.
phage P. simiae Grant Run MOI 10 -0.7 N.D. +0.2 -0.8 N.D.
rhizosphere rhizosphere sample 2; outgrowth in LB +0.2 N.D. -0.6 -1.0 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.9 N.D. +0.3 -0.7 N.D.
stress Thallium(I) acetate 0.0625 mg/ml -0.6 N.D. +0.1 -0.7 N.D.
stress Lomefloxacin 0.0001 mM -1.3 N.D. +0.1 -0.1 N.D.
soil soil sample 3; outgrowth in LB +0.7 N.D. -0.3 -1.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.6 N.D. -0.3 -0.7 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.9 N.D. +0.0 +0.9 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.7 N.D. +0.1 -0.7 N.D.
carbon source L-Valine (C) -0.6 N.D. -0.3 +0.9 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.8 N.D. -0.2 -0.5 N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 N.D. +0.1 +1.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +1.9 N.D. -0.1 -0.6 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.2 N.D. -0.0 +0.3 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.5 N.D. -0.1 +1.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.5 N.D. +0.3 +0.7 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.1 N.D. +0.5 +1.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +3.6 N.D. +0.0 +0.1 N.D.
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