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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_01015 and PS417_01020 overlap by 20 nucleotides
PS417_01020 and PS417_01025 overlap by 4 nucleotides
PS417_01025 and PS417_01030 overlap by 4 nucleotides
PS417_01030 and PS417_01035 are separated by 99 nucleotides
PS417_01015: PS417_01015 - polar amino acid ABC transporter permease, at 221,869 to 222,534
_01015
PS417_01020: PS417_01020 - glutamine ABC transporter permease, at 222,515 to 223,174
_01020
PS417_01025: PS417_01025 - glutamine ABC transporter ATP-binding protein, at 223,171 to 223,947
_01025
PS417_01030: PS417_01030 - glutamine ABC transporter substrate-binding protein, at 223,944 to 224,798
_01030
PS417_01035: PS417_01035 - ATP-binding protein, at 224,898 to 226,058
_01035
Group
Condition
PS417
_01015
PS417
_01020
PS417
_01025
PS417
_01030
PS417
_01035
phage
JP1 MOI 10
-1.2
-0.4
N.D.
-2.3
+0.4
phage
P. simiae Grant Run MOI 10
-0.3
-0.6
N.D.
-1.4
-0.6
phage
P. simiae OR Antrim MOI 10
-0.2
-0.5
N.D.
-1.9
-0.3
phage
JP1 MOI 1
-0.8
-0.3
N.D.
-0.8
-0.8
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.1
+0.0
N.D.
-2.5
-0.1
carbon source
L-Valine (C)
+0.1
-0.3
N.D.
-1.1
-1.0
liquid stress
Scopoletin 2 mM; liquid stress
+0.1
-0.3
N.D.
-1.8
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
-0.4
N.D.
-1.9
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
-0.4
N.D.
-1.8
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
-0.2
N.D.
-1.8
+0.2
soil
soil sample 3; outgrowth in LB
+0.1
+0.5
N.D.
-1.6
-0.3
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.0
+0.8
N.D.
-1.3
-0.4
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.1
+0.5
N.D.
-1.3
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.7
+0.4
N.D.
-0.8
+0.3
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.6
-0.3
N.D.
-1.0
-0.1
stress
methylglyoxal 0.032 vol%
+0.2
+0.6
N.D.
-1.3
+0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.2
+0.3
N.D.
-1.3
+0.4
root
root sample 6; outgrowth in LB
+0.9
+0.5
N.D.
-1.3
-0.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.9
-0.0
N.D.
-0.9
+0.4
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.4
+0.4
N.D.
-0.7
+0.5
phage
P. simiae OR Antrim MOI 0.1
-0.4
-0.3
N.D.
+1.5
+0.0
carbon source
2'-Deoxyinosine 5 mM (C)
+0.5
+0.6
N.D.
-0.9
+0.7
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.3
+1.3
N.D.
-0.6
+0.1
rhizosphere
rhizosphere sample 1; outgrowth in LB
+0.5
+1.2
N.D.
+0.4
-0.9
phage
JP1 MOI 0.1
-0.0
-0.2
N.D.
+1.7
+0.1
seeds
Growth on radish seeds for 24 hours
-0.1
+0.6
N.D.
+1.5
-0.0
soil
soil sample 5; outgrowth in LB
+0.8
+0.8
N.D.
+0.8
-0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.4
+0.4
N.D.
+0.6
+1.0
seeds
Growth on radish seeds for 72 hours
+0.3
+0.3
N.D.
+1.5
+0.4
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days
+0.1
-0.3
N.D.
+3.3
+0.1
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