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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_00090 and PS417_00095 are separated by 69 nucleotides
PS417_00095 and PS417_00100 are separated by 135 nucleotides
PS417_00100 and PS417_00110 are separated by 1212 nucleotides
PS417_00110 and PS417_00115 are separated by 130 nucleotides
PS417_00090: PS417_00090 - methionyl-tRNA formyltransferase, at 20,029 to 20,982
_00090
PS417_00095: PS417_00095 - peptide deformylase, at 21,052 to 21,558
_00095
PS417_00100: PS417_00100 - peptidoglycan-binding protein, at 21,694 to 22,470
_00100
PS417_00110: PS417_00110 - tRNA threonylcarbamoyladenosine biosynthesis protein RimN, at 23,683 to 24,240
_00110
PS417_00115: PS417_00115 - quinone oxidoreductase, at 24,371 to 25,348
_00115
Group
Condition
PS417
_00090
PS417
_00095
PS417
_00100
PS417
_00110
PS417
_00115
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
-0.3
N.D.
-1.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
-1.0
N.D.
-0.4
phage
P. simiae OR Antrim MOI 1
N.D.
N.D.
-0.3
N.D.
-0.8
seeds
Growth on radish seeds for 72 hours
N.D.
N.D.
-0.6
N.D.
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
-0.4
N.D.
-0.6
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
N.D.
-0.5
N.D.
-0.5
stress
Cisplatin 0.01 mg/ml
N.D.
N.D.
-0.6
N.D.
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
-0.6
N.D.
-0.4
phage
JP1 MOI 1
N.D.
N.D.
-0.6
N.D.
-0.3
soil
soil sample 3; outgrowth in LB
N.D.
N.D.
-0.7
N.D.
-0.2
motility
outer cut, LB soft agar motility assay
N.D.
N.D.
-0.8
N.D.
-0.1
phage
Wcs_1
N.D.
N.D.
-0.1
N.D.
-0.8
supernatant control
Vogels_fungal_media 0.1X
N.D.
N.D.
-0.5
N.D.
-0.3
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X
N.D.
N.D.
-0.6
N.D.
-0.2
motility
outer cut, LB soft agar motility assay
N.D.
N.D.
-0.3
N.D.
-0.5
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
N.D.
N.D.
+0.2
N.D.
-0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-tyrosine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
N.D.
N.D.
+0.3
N.D.
-0.6
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
N.D.
-0.5
N.D.
+0.3
phage
JP1 MOI 1
N.D.
N.D.
-0.6
N.D.
+0.4
stress
methylglyoxal 0.032 vol%
N.D.
N.D.
-0.4
N.D.
+0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
-0.4
N.D.
+0.4
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
N.D.
+0.7
N.D.
-0.4
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
N.D.
+0.7
N.D.
-0.2
phage
P. simiae ORA MOI 1
N.D.
N.D.
+0.7
N.D.
-0.1
phage
JP1 MOI 0.1
N.D.
N.D.
+0.7
N.D.
+0.1
solid stress
Fraxetin 1 mM; solid stress
N.D.
N.D.
+0.4
N.D.
+0.4
in planta
Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
N.D.
N.D.
+0.2
N.D.
+0.7
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
N.D.
+0.5
N.D.
+0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
+0.4
N.D.
+0.6
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
N.D.
+0.1
N.D.
+0.9
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