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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_00975 and PS417_00985 are separated by 136 nucleotides
PS417_00985 and PS417_00990 are separated by 176 nucleotides
PS417_00990 and PS417_00995 are separated by 226 nucleotides
PS417_00995 and PS417_01000 are separated by 50 nucleotides
PS417_00975: PS417_00975 - hypothetical protein, at 216,039 to 216,356
_00975
PS417_00985: PS417_00985 - MFS transporter, at 216,493 to 217,662
_00985
PS417_00990: PS417_00990 - LysR family transcriptional regulator, at 217,839 to 218,690
_00990
PS417_00995: PS417_00995 - alpha/beta hydrolase, at 218,917 to 219,498
_00995
PS417_01000: PS417_01000 - Fis family transcriptional regulator, at 219,549 to 220,475
_01000
Group
Condition
PS417
_00975
PS417
_00985
PS417
_00990
PS417
_00995
PS417
_01000
agar plate interaction control
Taped volatile agar plate with no fungus
-0.9
+0.0
-1.0
-1.7
-0.1
motility
inner cut, LB soft agar motility assay
-1.9
-0.9
-0.5
+0.2
+0.1
soil
soil sample 3; outgrowth in LB
-0.4
-0.3
-0.2
-2.0
+0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.4
-0.0
-0.7
-2.1
-0.3
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-1.6
-0.4
+0.2
-0.6
+0.0
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-2.0
-0.4
-0.3
-0.0
+0.4
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-1.2
-0.6
+0.1
-0.5
+0.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.3
+0.1
+0.4
-0.2
-0.9
stress
Gentamicin 0.008 mg/ml
-0.8
+0.0
+0.3
-1.8
+0.6
supernatant
Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
-1.3
+0.3
+0.0
-0.6
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.9
+0.6
-0.9
-0.3
+0.1
carbon source
Carbon source D-Mannitol 5 mM
+0.5
+0.2
-0.3
-1.0
-0.4
stress
methylglyoxal 0.032 vol%
-0.6
-0.3
+0.3
+0.7
-0.8
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Aspartic Acid; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-1.3
-0.3
-0.0
+0.5
+0.4
phage
P. simiae OR1 MOI 0.1
-1.2
-0.7
-0.6
+2.0
-0.1
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.3
-0.1
+0.5
-0.7
+1.1
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
-1.3
+0.1
-0.0
+0.8
+0.3
soil
soil sample 7; outgrowth in LB
+0.7
-1.0
+0.4
-0.1
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.0
+0.5
+0.6
-0.0
-0.3
rhizosphere
rhizosphere sample 1; outgrowth in LB
+1.2
-0.4
-0.3
+0.4
-0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.7
+0.2
+0.5
+0.9
+0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-1.0
+0.9
-0.0
+0.9
+0.1
carbon source
2-Deoxy-D-Ribose 10 mM (C)
+0.6
-0.6
-0.1
+1.0
+0.3
phage
Wcs_1
+1.4
-0.4
+0.2
+0.4
+0.2
seeds
Growth on radish seeds for 72 hours
+2.2
-0.7
+0.9
-0.3
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
-0.6
+0.4
+1.5
+0.3
soil
soil sample 4; outgrowth in LB
+1.6
+0.2
-0.2
+0.3
+0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.7
-0.1
+0.8
+0.9
+0.1
carbon source
2'-Deoxyinosine 5 mM (C)
+1.5
-0.2
+0.2
+1.4
-0.1
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+1.6
+0.1
-0.6
+1.5
+0.3
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