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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_09970 and PS417_09975 are separated by 270 nucleotides
PS417_09975 and PS417_09980 are separated by 76 nucleotides
PS417_09980 and PS417_09985 are separated by 4 nucleotides
PS417_09985 and PS417_09990 are separated by 83 nucleotides
PS417_09970: PS417_09970 - hypothetical protein, at 2,148,853 to 2,149,314
_09970
PS417_09975: PS417_09975 - hypothetical protein, at 2,149,585 to 2,149,893
_09975
PS417_09980: PS417_09980 - peptidase, at 2,149,970 to 2,150,197
_09980
PS417_09985: PS417_09985 - propanediol utilization protein, at 2,150,202 to 2,151,062
_09985
PS417_09990: PS417_09990 - regulatory protein, at 2,151,146 to 2,151,385
_09990
Group
Condition
PS417
_09970
PS417
_09975
PS417
_09980
PS417
_09985
PS417
_09990
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.7
-0.4
-0.3
N.D.
N.D.
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
-0.2
-0.5
-0.6
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.6
-0.3
-0.2
N.D.
N.D.
phage
Wcs_1
-0.2
-0.5
-0.4
N.D.
N.D.
stress
methylglyoxal 0.032 vol%
+0.1
-0.5
-0.7
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.4
-0.3
-0.3
N.D.
N.D.
phage
JP1 MOI 0.1
-0.1
-0.2
-0.7
N.D.
N.D.
soil
soil sample 4; outgrowth in LB
-0.3
-0.2
-0.5
N.D.
N.D.
carbon source
Deoxyribonucleic from herring sperm 10 mg/mL (C)
-0.0
-0.6
-0.4
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.0
-0.5
-0.4
N.D.
N.D.
seeds
Growth on radish seeds for 72 hours
+0.2
-0.7
-0.4
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.0
-0.8
+0.1
N.D.
N.D.
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.0
-1.0
+0.4
N.D.
N.D.
carbon source
Carbon source D-Mannitol 10 mM
+0.3
-0.4
-0.4
N.D.
N.D.
soil
soil sample 3; outgrowth in LB
-0.3
+0.2
-0.4
N.D.
N.D.
carbon source
L-Valine (C)
+0.2
-0.9
+0.4
N.D.
N.D.
soil
soil sample 2; outgrowth in LB
-0.2
+0.5
-0.5
N.D.
N.D.
soil
soil sample 8; outgrowth in LB
+0.3
+0.2
-0.7
N.D.
N.D.
phage
P. simiae Grant Run MOI 0.1
+0.2
+0.3
-0.6
N.D.
N.D.
carbon source
2'-Deoxyinosine 5 mM (C)
-0.2
+0.5
-0.5
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
+0.4
-0.7
N.D.
N.D.
agar plate interaction control
Taped volatile agar plate with no fungus
-0.4
+0.5
-0.0
N.D.
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.2
+0.8
-0.2
N.D.
N.D.
rhizosphere
rhizosphere sample 8; outgrowth in LB
-0.1
+1.0
-0.4
N.D.
N.D.
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.5
-0.3
+0.5
N.D.
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.1
-0.1
+0.9
N.D.
N.D.
supernatant control:fungal media
R2A with Vogels_fungal_media 0.8X
+0.2
+0.5
+0.3
N.D.
N.D.
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.2
+0.7
+0.3
N.D.
N.D.
phage
P. simiae ORA MOI 0.1
+0.5
+0.6
+0.2
N.D.
N.D.
rhizosphere
rhizosphere sample 1; outgrowth in LB
-0.0
+1.6
-0.2
N.D.
N.D.
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