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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_00965 and PS417_00970 are separated by 82 nucleotides
PS417_00970 and PS417_00975 are separated by 15 nucleotides
PS417_00975 and PS417_00985 are separated by 136 nucleotides
PS417_00985 and PS417_00990 are separated by 176 nucleotides
PS417_00965: PS417_00965 - AraC family transcriptional regulator, at 214,157 to 215,161
_00965
PS417_00970: PS417_00970 - hypothetical protein, at 215,244 to 216,023
_00970
PS417_00975: PS417_00975 - hypothetical protein, at 216,039 to 216,356
_00975
PS417_00985: PS417_00985 - MFS transporter, at 216,493 to 217,662
_00985
PS417_00990: PS417_00990 - LysR family transcriptional regulator, at 217,839 to 218,690
_00990
Group
Condition
PS417
_00965
PS417
_00970
PS417
_00975
PS417
_00985
PS417
_00990
phage
P. simiae OR1 MOI 0.1
-0.1
-0.4
-1.2
-0.7
-0.6
motility
inner cut, LB soft agar motility assay
+0.4
+0.0
-1.9
-0.9
-0.5
phage
JP1 MOI 10
-0.0
-0.7
-0.5
-1.2
-0.1
supernatant control
Vogels_fungal_media 0.1X
-0.1
-0.6
-0.5
-0.5
-0.8
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.6
-0.1
-1.2
-0.6
+0.1
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.1
+0.2
-2.0
-0.4
-0.3
motility
inner cut, LB soft agar motility assay
-0.5
-0.3
-0.7
-0.1
-0.5
agar plate interaction control
Taped volatile agar plate with no fungus
-0.4
+0.2
-0.9
+0.0
-1.0
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
-0.7
+0.1
-0.1
-0.7
-0.6
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+0.1
+0.2
-1.6
-0.4
+0.2
motility
outer cut, LB soft agar motility assay
-0.5
+0.3
-0.5
-0.8
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
+0.1
-1.5
-0.1
+0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.3
+0.1
-1.3
+0.1
+0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.5
+0.7
-0.9
+0.6
-0.9
agar plate interaction control
Taped volatile agar plate with no fungus
+0.3
-0.2
-1.1
-0.3
+0.4
soil
soil sample 7; outgrowth in LB
+0.0
-0.4
+0.7
-1.0
+0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
+0.1
+0.9
-0.6
-0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.1
+0.5
-1.0
+0.9
-0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
+0.3
-1.0
+0.5
+0.6
carbon source
Xylitol (C)
-0.2
-0.2
+1.2
-0.3
+0.3
rhizosphere
rhizosphere sample 1; outgrowth in LB
+0.2
+0.0
+1.2
-0.4
-0.3
rhizosphere
rhizosphere sample 8; outgrowth in LB
+0.6
-0.3
+0.9
-0.3
-0.0
phage
Wcs_1
-0.2
-0.1
+1.4
-0.4
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.3
+0.2
+0.9
-0.6
+0.3
carbon source
2'-Deoxyinosine 5 mM (C)
-0.3
+0.0
+1.5
-0.2
+0.2
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.1
+0.2
+1.6
+0.1
-0.6
soil
soil sample 4; outgrowth in LB
+0.4
-0.2
+1.6
+0.2
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
+0.6
+0.8
+0.4
+0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Aspartic Acid; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.4
-0.1
+0.9
+0.4
+0.7
seeds
Growth on radish seeds for 72 hours
+0.0
-0.0
+2.2
-0.7
+0.9
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