Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_09795 and PS417_09800 are separated by 8 nucleotidesPS417_09800 and PS417_09805 are separated by 27 nucleotidesPS417_09805 and PS417_09810 overlap by 4 nucleotidesPS417_09810 and PS417_09815 are separated by 191 nucleotides PS417_09795: PS417_09795 - lipase, at 2,123,483 to 2,125,633 _09795 PS417_09800: PS417_09800 - cytosine deaminase, at 2,125,642 to 2,126,877 _09800 PS417_09805: PS417_09805 - CdaR family transcriptional regulator, at 2,126,905 to 2,128,110 _09805 PS417_09810: PS417_09810 - cytosine permease, at 2,128,107 to 2,129,375 _09810 PS417_09815: PS417_09815 - DNA mismatch repair protein MutT, at 2,129,567 to 2,130,190 _09815
Group Condition PS417_09795 PS417_09800 PS417_09805 PS417_09810 PS417_09815
nitrogen source Cytidine (N) +0.1 -3.1 -1.1 -2.0 -0.2
nitrogen source Cytosine (N) -0.0 -2.0 -0.9 -1.3 -0.2
phage P. simiae ORA MOI 0.1 -0.1 -0.4 -0.8 -0.3 -0.1
phage P. simiae ORA MOI 0.1 -0.2 +0.0 -0.6 -0.3 -0.4
phage P. simiae Grant Run MOI 0.1 -0.2 -0.6 -0.5 -0.4 +0.2
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 -0.4 -0.4 -0.1 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 -0.6 -0.5 -0.3 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 -0.2 -0.6 +0.1 -0.3
phage P. simiae ORA MOI 1 -0.1 -0.6 -0.3 -0.1 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 -0.8 -0.4 +0.1 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.0 -0.9 +0.3 -0.3 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.0 -0.9 -0.1 -0.1 +0.2
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.1 -0.3 -0.4 -0.3 +0.1
motility outer cut, LB soft agar motility assay -0.2 -0.4 -0.4 -0.1 +0.3
root root sample 6; outgrowth in LB -0.4 -0.2 -0.3 +0.4 -0.3
soil soil sample 2; outgrowth in LB -0.1 +0.1 +0.3 -0.5 -0.5
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.2 -0.8 -0.1 -0.2 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 -0.4 -0.3 +0.3 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 -0.7 +0.2 -0.2 +0.3
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x +0.1 +0.4 -0.5 -0.1 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 -0.6 -0.4 +0.3 +0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.1 -0.6 +0.1 +0.4 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 -0.1 -0.7 +0.4 +0.2
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.2 +0.2 -0.5 -0.3 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.1 +0.8 -0.4 -0.0 -0.3
phage P. simiae OR Antrim MOI 1 -0.1 +0.9 -0.6 +0.1 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.2 -0.4 +0.6 +0.4 -0.2
solid stress Fraxetin 2 mM; solid stress +0.1 +0.5 -0.5 +0.1 +0.2
agar plate interaction control Taped volatile agar plate with no fungus -0.0 +0.3 +0.9 -0.2 +0.2
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 +1.0 -0.3 +0.0 +0.5
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