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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_09795 and PS417_09800 are separated by 8 nucleotides
PS417_09800 and PS417_09805 are separated by 27 nucleotides
PS417_09805 and PS417_09810 overlap by 4 nucleotides
PS417_09810 and PS417_09815 are separated by 191 nucleotides
PS417_09795: PS417_09795 - lipase, at 2,123,483 to 2,125,633
_09795
PS417_09800: PS417_09800 - cytosine deaminase, at 2,125,642 to 2,126,877
_09800
PS417_09805: PS417_09805 - CdaR family transcriptional regulator, at 2,126,905 to 2,128,110
_09805
PS417_09810: PS417_09810 - cytosine permease, at 2,128,107 to 2,129,375
_09810
PS417_09815: PS417_09815 - DNA mismatch repair protein MutT, at 2,129,567 to 2,130,190
_09815
Group
Condition
PS417
_09795
PS417
_09800
PS417
_09805
PS417
_09810
PS417
_09815
nitrogen source
Cytidine (N)
+0.1
-3.1
-1.1
-2.0
-0.2
nitrogen source
Cytosine (N)
-0.0
-2.0
-0.9
-1.3
-0.2
phage
P. simiae ORA MOI 0.1
-0.1
-0.4
-0.8
-0.3
-0.1
phage
P. simiae ORA MOI 0.1
-0.2
+0.0
-0.6
-0.3
-0.4
phage
P. simiae Grant Run MOI 0.1
-0.2
-0.6
-0.5
-0.4
+0.2
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.1
-0.4
-0.4
-0.1
-0.5
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.1
-0.6
-0.5
-0.3
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.3
-0.2
-0.6
+0.1
-0.3
phage
P. simiae ORA MOI 1
-0.1
-0.6
-0.3
-0.1
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
-0.8
-0.4
+0.1
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.0
-0.9
+0.3
-0.3
-0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.0
-0.9
-0.1
-0.1
+0.2
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.1
-0.3
-0.4
-0.3
+0.1
motility
outer cut, LB soft agar motility assay
-0.2
-0.4
-0.4
-0.1
+0.3
root
root sample 6; outgrowth in LB
-0.4
-0.2
-0.3
+0.4
-0.3
soil
soil sample 2; outgrowth in LB
-0.1
+0.1
+0.3
-0.5
-0.5
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.2
-0.8
-0.1
-0.2
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
-0.4
-0.3
+0.3
-0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.1
-0.7
+0.2
-0.2
+0.3
supernatant
RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x
+0.1
+0.4
-0.5
-0.1
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
-0.6
-0.4
+0.3
+0.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.1
-0.6
+0.1
+0.4
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
-0.1
-0.7
+0.4
+0.2
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.2
+0.2
-0.5
-0.3
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.1
+0.8
-0.4
-0.0
-0.3
phage
P. simiae OR Antrim MOI 1
-0.1
+0.9
-0.6
+0.1
-0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
-0.2
-0.4
+0.6
+0.4
-0.2
solid stress
Fraxetin 2 mM; solid stress
+0.1
+0.5
-0.5
+0.1
+0.2
agar plate interaction control
Taped volatile agar plate with no fungus
-0.0
+0.3
+0.9
-0.2
+0.2
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.2
+1.0
-0.3
+0.0
+0.5
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