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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_09740 and PS417_09745 are separated by 17 nucleotides
PS417_09745 and PS417_09750 are separated by 97 nucleotides
PS417_09750 and PS417_09755 are separated by 500 nucleotides
PS417_09755 and PS417_09765 are separated by 651 nucleotides
PS417_09740: PS417_09740 - RNA polymerase subunit sigma-24, at 2,110,311 to 2,110,811
_09740
PS417_09745: PS417_09745 - peptide ABC transporter substrate-binding protein, at 2,110,829 to 2,111,779
_09745
PS417_09750: PS417_09750 - amino acid ABC transporter substrate-binding protein, at 2,111,877 to 2,114,282
_09750
PS417_09755: PS417_09755 - hypothetical protein, at 2,114,783 to 2,115,316
_09755
PS417_09765: PS417_09765 - hypothetical protein, at 2,115,968 to 2,116,879
_09765
Group
Condition
PS417
_09740
PS417
_09745
PS417
_09750
PS417
_09755
PS417
_09765
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.7
-1.1
-0.1
-0.5
+0.2
phage
P. simiae OR1 MOI 0.1
-1.3
-0.0
-0.3
-0.3
-0.1
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.8
-0.6
-0.1
-0.4
-0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.8
-0.8
-0.1
+0.1
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.2
-0.7
-0.2
+0.0
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.6
-0.9
+0.0
-0.1
-0.3
carbon source
2-Deoxy-D-ribonic lithium salt 10 mM (C)
-1.3
-0.1
-0.0
-0.4
+0.0
agar plate interaction control
Taped volatile agar plate with no fungus
-0.2
-1.0
+0.0
-0.5
+0.0
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.5
-0.7
-0.1
-0.2
+0.1
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.1
-0.2
-0.2
+0.3
-0.1
rhizosphere
rhizosphere sample 1; outgrowth in LB
-0.8
-0.3
-0.2
+0.5
-0.3
stress
Doxycycline hyclate 0.001 mg/ml
-0.5
-0.4
+0.0
-0.4
+0.3
liquid stress
Scopoletin 3 mM; liquid stress
-0.8
-0.7
+0.0
+0.1
+0.4
soil
soil sample 3; outgrowth in LB
-0.8
-0.4
+0.0
+0.1
+0.3
soil
soil sample 4; outgrowth in LB
-0.7
-0.9
+0.0
+1.0
-0.1
soil
soil sample 5; outgrowth in LB
-0.5
-0.5
+0.1
+0.6
-0.4
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.5
-0.6
+0.1
+0.2
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
-0.7
-0.2
+0.4
+0.4
carbon source
L-Valine (C)
+0.7
-0.6
+0.1
-0.3
+0.1
in planta
Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.4
+0.8
+0.1
-0.2
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.8
-0.6
+0.2
-0.4
+0.4
motility
inner cut, LB soft agar motility assay
+0.6
-0.5
-0.0
+0.3
+0.2
motility
inner cut, LB soft agar motility assay
+0.5
-0.5
+0.1
+0.3
+0.2
stress
1-ethyl-3-methylimidazolium acetate 35 mM
+0.8
+0.3
+0.0
-0.6
+0.3
phage
P. simiae Grant Run MOI 10
+0.0
+0.5
+0.1
+0.6
-0.4
root
root sample 6; outgrowth in LB
+1.2
-0.6
-0.2
+0.7
-0.1
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+1.4
+0.1
-0.1
-0.3
-0.0
seeds
Growth on radish seeds for 72 hours
+0.5
+0.9
-0.1
+0.0
-0.2
rhizosphere
rhizosphere sample 2; outgrowth in LB
+0.4
-0.3
+0.1
+0.8
+0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-0.1
+1.3
-0.1
+0.4
+0.3
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