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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_00085 and PS417_00090 overlap by 4 nucleotides
PS417_00090 and PS417_00095 are separated by 69 nucleotides
PS417_00095 and PS417_00100 are separated by 135 nucleotides
PS417_00100 and PS417_00110 are separated by 1212 nucleotides
PS417_00085: PS417_00085 - 16S rRNA methyltransferase, at 18,722 to 20,032
_00085
PS417_00090: PS417_00090 - methionyl-tRNA formyltransferase, at 20,029 to 20,982
_00090
PS417_00095: PS417_00095 - peptide deformylase, at 21,052 to 21,558
_00095
PS417_00100: PS417_00100 - peptidoglycan-binding protein, at 21,694 to 22,470
_00100
PS417_00110: PS417_00110 - tRNA threonylcarbamoyladenosine biosynthesis protein RimN, at 23,683 to 24,240
_00110
Group
Condition
PS417
_00085
PS417
_00090
PS417
_00095
PS417
_00100
PS417
_00110
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-2.7
N.D.
N.D.
-0.1
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-2.6
N.D.
N.D.
-0.1
N.D.
supernatant control
Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
-2.0
N.D.
N.D.
-0.6
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.4
N.D.
N.D.
-1.0
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-2.2
N.D.
N.D.
-0.2
N.D.
agar plate interaction
Taped volatile agar plate with Trichoderma atroviridae IMI
-2.0
N.D.
N.D.
-0.4
N.D.
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.9
N.D.
N.D.
-0.4
N.D.
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
-2.1
N.D.
N.D.
-0.0
N.D.
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-1.5
N.D.
N.D.
-0.5
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-2.0
N.D.
N.D.
-0.0
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-1.8
N.D.
N.D.
-0.1
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-2.3
N.D.
N.D.
+0.5
N.D.
soil
soil sample 5; outgrowth in LB
-1.9
N.D.
N.D.
+0.0
N.D.
nophagecontrol
Only library
-1.5
N.D.
N.D.
-0.4
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.5
N.D.
N.D.
-0.4
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.1
N.D.
N.D.
-0.6
N.D.
motility
outer cut, LB soft agar motility assay
-0.9
N.D.
N.D.
-0.8
N.D.
seeds
Growth on radish seeds for 72 hours
-1.6
N.D.
N.D.
-0.0
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.5
N.D.
N.D.
-0.1
N.D.
phage
JP1 MOI 10
-1.5
N.D.
N.D.
-0.0
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days
-1.8
N.D.
N.D.
+0.3
N.D.
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-1.2
N.D.
N.D.
-0.3
N.D.
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X
-0.9
N.D.
N.D.
-0.6
N.D.
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-1.7
N.D.
N.D.
+0.4
N.D.
soil
soil sample 8; outgrowth in LB
-1.4
N.D.
N.D.
+0.2
N.D.
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-1.5
N.D.
N.D.
+0.7
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.1
N.D.
N.D.
+0.3
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-1.1
N.D.
N.D.
+0.4
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-1.1
N.D.
N.D.
+0.5
N.D.
phage
JP1 MOI 1
+1.0
N.D.
N.D.
-0.6
N.D.
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