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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_09485 and PS417_09490 are separated by 115 nucleotides
PS417_09490 and PS417_09495 overlap by 4 nucleotides
PS417_09495 and PS417_09500 are separated by 35 nucleotides
PS417_09500 and PS417_09505 are separated by 111 nucleotides
PS417_09485: PS417_09485 - LysR family transcriptional regulator, at 2,054,245 to 2,055,153
_09485
PS417_09490: PS417_09490 - quinone oxidoreductase, at 2,055,269 to 2,056,255
_09490
PS417_09495: PS417_09495 - DSBA oxidoreductase, at 2,056,252 to 2,056,842
_09495
PS417_09500: PS417_09500 - glucose 1-dehydrogenase, at 2,056,878 to 2,057,609
_09500
PS417_09505: PS417_09505 - hypothetical protein, at 2,057,721 to 2,058,002
_09505
Group
Condition
PS417
_09485
PS417
_09490
PS417
_09495
PS417
_09500
PS417
_09505
rhizosphere
rhizosphere sample 2; outgrowth in LB
-0.4
-0.0
-0.2
-0.1
-1.1
carbon source
L-Valine (C)
-0.2
-0.2
-0.3
-0.3
-0.6
soil
soil sample 4; outgrowth in LB
-0.1
+0.1
-0.5
-0.4
-0.7
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.3
-0.2
+0.2
-0.3
-0.6
soil
soil sample 3; outgrowth in LB
-0.3
+0.2
-0.5
-0.4
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
-0.2
+0.1
-1.0
+0.4
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.0
-0.2
+0.2
+0.1
-0.9
soil
soil sample 2; outgrowth in LB
+0.4
-0.3
-0.1
+0.0
-0.8
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.3
+0.2
-0.2
-0.0
-1.0
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.3
-0.2
-0.3
+0.4
-0.3
phage
P. simiae ORA MOI 0.1
-0.2
-0.4
-0.3
+0.2
+0.3
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.2
-0.2
-0.3
+0.5
-0.5
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.2
+0.6
-0.4
+0.1
-0.4
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.1
-0.2
+0.7
+0.4
-0.6
seeds
Growth on radish seeds for 72 hours
-0.1
+0.3
-0.5
-0.1
+0.7
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
-0.3
-0.3
-0.3
+1.0
stress
Gentamicin 0.008 mg/ml
+0.5
+0.4
-0.0
+0.1
-0.6
carbon source
octanoate (C)
-0.1
-0.1
+0.1
-0.1
+1.1
agar plate interaction control
Taped volatile agar plate with no fungus
-0.1
-0.2
+0.3
+0.5
+0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
-0.1
+0.5
-0.3
+0.7
phage
P. simiae Grant Run MOI 10
-0.2
-0.1
+0.3
+0.3
+0.8
phage
P. simiae ORA MOI 1
-0.0
-0.2
+0.3
+0.1
+1.0
carbon source
octanoate (C)
+0.1
-0.2
+0.1
+0.1
+1.1
stress
Nalidixic 0.015 mg/ml
+0.4
+0.1
-0.0
+0.2
+0.8
stress
Doxycycline hyclate 0.001 mg/ml
+0.0
+0.1
-0.4
+0.4
+1.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.0
-0.1
+0.7
+0.3
+0.7
phage
P. simiae OR Antrim MOI 10
+0.2
+0.1
+0.4
+0.4
+0.6
stress
Tetracycline 0.001 mg/ml
+0.2
+0.1
+0.1
+0.2
+1.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.5
+0.5
+0.9
-0.0
+0.0
stress
Neomycin 0.04 mg/ml
+0.0
+0.2
+0.3
+0.2
+2.7
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