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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_09475 and PS417_09480 are separated by 49 nucleotides
PS417_09480 and PS417_09485 overlap by 8 nucleotides
PS417_09485 and PS417_09490 are separated by 115 nucleotides
PS417_09490 and PS417_09495 overlap by 4 nucleotides
PS417_09475: PS417_09475 - class V aminotransferase, at 2,051,234 to 2,052,394
_09475
PS417_09480: PS417_09480 - peptidase M24, at 2,052,444 to 2,054,252
_09480
PS417_09485: PS417_09485 - LysR family transcriptional regulator, at 2,054,245 to 2,055,153
_09485
PS417_09490: PS417_09490 - quinone oxidoreductase, at 2,055,269 to 2,056,255
_09490
PS417_09495: PS417_09495 - DSBA oxidoreductase, at 2,056,252 to 2,056,842
_09495
Group
Condition
PS417
_09475
PS417
_09480
PS417
_09485
PS417
_09490
PS417
_09495
phage
JP1 MOI 1
-0.9
-0.2
-0.5
-0.1
-0.2
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.5
-0.4
-0.1
-0.4
-0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.7
-0.3
-0.5
+0.1
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.7
-0.3
-0.1
-0.1
-0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.6
-0.3
+0.2
-0.3
-0.3
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.6
+0.2
-0.3
-0.2
-0.3
phage
P. simiae ORA MOI 0.1
-0.4
+0.1
-0.2
-0.4
-0.3
phage
JP1 MOI 10
-0.9
+0.3
+0.0
-0.0
-0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.9
-0.3
-0.0
+0.3
-0.1
seeds
Growth on radish seeds for 72 hours
-0.6
+0.0
-0.1
+0.3
-0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.5
-0.3
-0.5
-0.2
-0.1
carbon source
2-Deoxy-D-Ribose 10 mM (C)
-0.6
+0.1
-0.0
-0.4
+0.3
supernatant control
Vogels_fungal_media 0.1X
+0.6
-0.3
+0.0
-0.5
-0.2
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.6
+0.2
+0.1
+0.4
-0.4
rhizosphere
rhizosphere sample 2; outgrowth in LB
+0.7
-0.2
-0.4
-0.0
-0.2
phage
JP1 MOI 0.1
-0.8
-0.1
+0.1
+0.0
+0.6
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.1
-0.3
+0.2
+0.6
-0.4
supernatant
Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
+0.8
-1.0
+0.0
-0.1
+0.1
soil
soil sample 3; outgrowth in LB
+0.5
+0.0
-0.3
+0.2
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
-0.5
+0.1
+0.2
+0.4
stress
Gentamicin 0.008 mg/ml
-0.5
-0.4
+0.5
+0.4
-0.0
phage
P. simiae OR Antrim MOI 10
-0.8
+0.2
+0.2
+0.1
+0.4
seeds
Growth on radish seeds for 24 hours
-0.6
+0.4
+0.2
+0.6
-0.3
rhizosphere
rhizosphere sample 8; outgrowth in LB
+0.3
+0.4
-0.3
+0.2
-0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.4
-0.3
+0.2
-0.3
+0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
-0.3
+0.0
-0.1
+0.7
seeds
Growth on radish seeds for 72 hours
-0.1
+0.4
-0.1
+0.9
-0.0
stress
Cobalt chloride 0.16 mM
+0.5
+0.4
-0.2
+0.1
+0.4
in planta
Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.8
-0.0
+0.5
+0.4
+0.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.1
-0.3
+0.5
+0.5
+0.9
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