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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_09420 and PS417_09425 are separated by 57 nucleotides
PS417_09425 and PS417_09430 are separated by 100 nucleotides
PS417_09430 and PS417_09435 are separated by 201 nucleotides
PS417_09435 and PS417_09440 are separated by 243 nucleotides
PS417_09420: PS417_09420 - LysR family transcriptional regulator, at 2,041,154 to 2,042,089
_09420
PS417_09425: PS417_09425 - C4-dicarboxylate transporter, at 2,042,147 to 2,043,460
_09425
PS417_09430: PS417_09430 - alpha/beta hydrolase, at 2,043,561 to 2,044,436
_09430
PS417_09435: PS417_09435 - glyoxalase, at 2,044,638 to 2,045,096
_09435
PS417_09440: PS417_09440 - MerR family transcriptional regulator, at 2,045,340 to 2,045,795
_09440
Group
Condition
PS417
_09420
PS417
_09425
PS417
_09430
PS417
_09435
PS417
_09440
root
root sample 6; outgrowth in LB
-1.3
+0.1
-0.4
-0.3
-2.1
solid stress
Fraxetin 2 mM; solid stress
-3.2
+0.0
+0.4
-0.2
-0.5
phage
JP1 MOI 0.1
+0.1
+0.1
-0.3
-0.8
-2.2
seeds
Growth on radish seeds for 72 hours
-0.4
+0.0
-0.5
-0.7
-1.4
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB
-1.4
-0.2
-0.3
-0.2
-0.4
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.6
-0.2
-0.5
+0.1
-1.2
in planta
Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-1.5
+0.0
-0.4
-0.0
-0.6
nophagecontrol
Only library
-2.0
-0.2
-0.3
-0.3
+0.4
liquid stress
Fraxetin 2 mM; liquid stress
-0.8
-0.1
-0.5
+0.1
-0.9
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X
-1.9
-0.2
+0.0
-0.0
+0.2
phage
P. simiae Grant Run MOI 0.1
-1.3
+0.1
-0.0
+0.4
-1.1
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.7
+0.5
+0.0
-0.3
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.7
-0.0
+0.4
-0.5
-0.7
soil
soil sample 8; outgrowth in LB
+0.8
-0.1
-0.2
-0.5
-1.5
seeds
Growth on radish seeds for 24 hours
-2.2
+0.1
+0.4
-0.1
+0.7
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-1.5
+0.1
-0.3
+0.1
+0.7
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.5
+0.1
+0.2
-0.1
-1.4
soil
soil sample 7; outgrowth in LB
+0.8
-0.4
-0.2
-0.5
-0.4
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.6
+0.1
-0.5
+1.0
-0.0
phage
P. simiae OR Antrim MOI 0.1
+1.2
+0.2
-0.2
-0.8
-0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.7
-0.1
+0.6
+0.2
-1.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.8
+0.3
+0.3
-0.2
-0.8
agar plate interaction
Taped volatile agar plate with Trichoderma atroviridae IMI
+0.7
+0.2
+0.5
+0.1
-0.9
seeds
Growth on radish seeds for 72 hours
+0.9
-0.5
-0.5
+0.9
+0.2
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.6
+0.1
-0.2
+0.8
+0.8
motility
inner cut, LB soft agar motility assay
+0.9
-0.1
+0.2
-0.5
+0.6
agar plate interaction control
Taped volatile agar plate with no fungus
+1.0
+0.2
-0.4
-0.1
+0.7
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+1.2
+0.2
+0.0
+0.4
-0.5
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.5
+0.1
-0.3
+1.3
-0.2
nitrogen source
Urea (N)
+0.1
+0.1
-0.2
+0.2
+1.7
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