Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_09420 and PS417_09425 are separated by 57 nucleotidesPS417_09425 and PS417_09430 are separated by 100 nucleotidesPS417_09430 and PS417_09435 are separated by 201 nucleotidesPS417_09435 and PS417_09440 are separated by 243 nucleotides PS417_09420: PS417_09420 - LysR family transcriptional regulator, at 2,041,154 to 2,042,089 _09420 PS417_09425: PS417_09425 - C4-dicarboxylate transporter, at 2,042,147 to 2,043,460 _09425 PS417_09430: PS417_09430 - alpha/beta hydrolase, at 2,043,561 to 2,044,436 _09430 PS417_09435: PS417_09435 - glyoxalase, at 2,044,638 to 2,045,096 _09435 PS417_09440: PS417_09440 - MerR family transcriptional regulator, at 2,045,340 to 2,045,795 _09440
Group Condition PS417_09420 PS417_09425 PS417_09430 PS417_09435 PS417_09440
root root sample 6; outgrowth in LB -1.3 +0.1 -0.4 -0.3 -2.1
solid stress Fraxetin 2 mM; solid stress -3.2 +0.0 +0.4 -0.2 -0.5
phage JP1 MOI 0.1 +0.1 +0.1 -0.3 -0.8 -2.2
seeds Growth on radish seeds for 72 hours -0.4 +0.0 -0.5 -0.7 -1.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB -1.4 -0.2 -0.3 -0.2 -0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.6 -0.2 -0.5 +0.1 -1.2
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -1.5 +0.0 -0.4 -0.0 -0.6
nophagecontrol Only library -2.0 -0.2 -0.3 -0.3 +0.4
liquid stress Fraxetin 2 mM; liquid stress -0.8 -0.1 -0.5 +0.1 -0.9
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X -1.9 -0.2 +0.0 -0.0 +0.2
phage P. simiae Grant Run MOI 0.1 -1.3 +0.1 -0.0 +0.4 -1.1
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.7 +0.5 +0.0 -0.3 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.7 -0.0 +0.4 -0.5 -0.7
soil soil sample 8; outgrowth in LB +0.8 -0.1 -0.2 -0.5 -1.5
seeds Growth on radish seeds for 24 hours -2.2 +0.1 +0.4 -0.1 +0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.5 +0.1 -0.3 +0.1 +0.7
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.5 +0.1 +0.2 -0.1 -1.4
soil soil sample 7; outgrowth in LB +0.8 -0.4 -0.2 -0.5 -0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.6 +0.1 -0.5 +1.0 -0.0
phage P. simiae OR Antrim MOI 0.1 +1.2 +0.2 -0.2 -0.8 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.7 -0.1 +0.6 +0.2 -1.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.8 +0.3 +0.3 -0.2 -0.8
agar plate interaction Taped volatile agar plate with Trichoderma atroviridae IMI +0.7 +0.2 +0.5 +0.1 -0.9
seeds Growth on radish seeds for 72 hours +0.9 -0.5 -0.5 +0.9 +0.2
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.6 +0.1 -0.2 +0.8 +0.8
motility inner cut, LB soft agar motility assay +0.9 -0.1 +0.2 -0.5 +0.6
agar plate interaction control Taped volatile agar plate with no fungus +1.0 +0.2 -0.4 -0.1 +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.2 +0.2 +0.0 +0.4 -0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 +0.1 -0.3 +1.3 -0.2
nitrogen source Urea (N) +0.1 +0.1 -0.2 +0.2 +1.7
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