Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_09225 and PS417_09230 are separated by 67 nucleotidesPS417_09230 and PS417_09235 are separated by 112 nucleotidesPS417_09235 and PS417_09240 are separated by 105 nucleotidesPS417_09240 and PS417_09245 are separated by 172 nucleotides PS417_09225: PS417_09225 - C4-dicarboxylate ABC transporter, at 2,003,586 to 2,004,734 _09225 PS417_09230: PS417_09230 - glycerol acyltransferase, at 2,004,802 to 2,006,676 _09230 PS417_09235: PS417_09235 - molecular chaperone, at 2,006,789 to 2,007,103 _09235 PS417_09240: PS417_09240 - sodium transporter, at 2,007,209 to 2,008,174 _09240 PS417_09245: PS417_09245 - recombinase RdgC, at 2,008,347 to 2,009,267 _09245
Group Condition PS417_09225 PS417_09230 PS417_09235 PS417_09240 PS417_09245
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 -0.3 -1.7 N.D. -5.3
stress methylglyoxal 0.032 vol% +0.2 +0.3 -4.7 N.D. -2.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.6 -0.1 -4.9 N.D. -0.6
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.1 +0.3 -4.6 N.D. -1.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 +0.1 -4.9 N.D. -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.0 -0.0 -0.8 N.D. -3.5
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.2 +0.2 -4.6 N.D. +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.0 -0.1 -0.4 N.D. -3.7
phage P. simiae Grant Run MOI 10 -0.5 -0.1 +0.6 N.D. -3.9
phage JP1 MOI 0.1 +0.1 -0.1 -2.9 N.D. -1.0
solid stress Fraxetin 2 mM; solid stress -0.1 +0.1 -0.6 N.D. -3.0
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.2 +0.1 -0.7 N.D. -2.4
soil soil sample 5; outgrowth in LB -0.6 +0.0 -2.4 N.D. -0.2
liquid stress 80% Methanol 3 mM; liquid stress -0.2 -0.0 -0.7 N.D. -2.1
solid stress Scopoletin 1 mM; solid stress -0.2 -0.0 -0.8 N.D. -2.0
solid stress Scopoletin 2 mM; solid stress -0.1 +0.1 -0.5 N.D. -2.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.1 +0.1 -0.7 N.D. -2.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.1 +0.1 -1.9 N.D. -1.0
carbon source RCH2_defined_glucose with MS_media 1x +0.1 -0.3 -1.9 N.D. -0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 -0.1 -2.7 N.D. +0.4
supernatant control Vogels_fungal_media 0.1X -0.4 +0.3 +0.2 N.D. -2.6
root root sample 6; outgrowth in LB +0.6 +0.2 -3.2 N.D. +1.1
solid stress Fraxetin 3 mM; solid stress -0.3 +0.1 +0.9 N.D. -2.0
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -2.0 -0.0 +0.6 N.D. +0.4
phage P. simiae OR Antrim MOI 10 +0.2 +0.2 +1.0 N.D. -2.4
agar plate interaction control Taped volatile agar plate with no fungus +0.6 -0.6 +0.9 N.D. -1.8
carbon source L-Valine (C) +0.1 -0.1 -1.8 N.D. +1.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.3 +0.3 +1.3 N.D. +1.2
seeds Growth on radish seeds for 72 hours +0.6 +0.0 +3.8 N.D. -0.9
seeds Growth on radish seeds for 72 hours -0.4 +0.1 +3.2 N.D. +0.8
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