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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_09225 and PS417_09230 are separated by 67 nucleotides
PS417_09230 and PS417_09235 are separated by 112 nucleotides
PS417_09235 and PS417_09240 are separated by 105 nucleotides
PS417_09240 and PS417_09245 are separated by 172 nucleotides
PS417_09225: PS417_09225 - C4-dicarboxylate ABC transporter, at 2,003,586 to 2,004,734
_09225
PS417_09230: PS417_09230 - glycerol acyltransferase, at 2,004,802 to 2,006,676
_09230
PS417_09235: PS417_09235 - molecular chaperone, at 2,006,789 to 2,007,103
_09235
PS417_09240: PS417_09240 - sodium transporter, at 2,007,209 to 2,008,174
_09240
PS417_09245: PS417_09245 - recombinase RdgC, at 2,008,347 to 2,009,267
_09245
Group
Condition
PS417
_09225
PS417
_09230
PS417
_09235
PS417
_09240
PS417
_09245
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
-0.3
-1.7
N.D.
-5.3
stress
methylglyoxal 0.032 vol%
+0.2
+0.3
-4.7
N.D.
-2.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.6
-0.1
-4.9
N.D.
-0.6
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.1
+0.3
-4.6
N.D.
-1.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.2
+0.1
-4.9
N.D.
-0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.0
-0.0
-0.8
N.D.
-3.5
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.2
+0.2
-4.6
N.D.
+0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.0
-0.1
-0.4
N.D.
-3.7
phage
P. simiae Grant Run MOI 10
-0.5
-0.1
+0.6
N.D.
-3.9
phage
JP1 MOI 0.1
+0.1
-0.1
-2.9
N.D.
-1.0
solid stress
Fraxetin 2 mM; solid stress
-0.1
+0.1
-0.6
N.D.
-3.0
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.2
+0.1
-0.7
N.D.
-2.4
soil
soil sample 5; outgrowth in LB
-0.6
+0.0
-2.4
N.D.
-0.2
liquid stress
80% Methanol 3 mM; liquid stress
-0.2
-0.0
-0.7
N.D.
-2.1
solid stress
Scopoletin 1 mM; solid stress
-0.2
-0.0
-0.8
N.D.
-2.0
solid stress
Scopoletin 2 mM; solid stress
-0.1
+0.1
-0.5
N.D.
-2.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.1
+0.1
-0.7
N.D.
-2.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.1
+0.1
-1.9
N.D.
-1.0
carbon source
RCH2_defined_glucose with MS_media 1x
+0.1
-0.3
-1.9
N.D.
-0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
-0.1
-2.7
N.D.
+0.4
supernatant control
Vogels_fungal_media 0.1X
-0.4
+0.3
+0.2
N.D.
-2.6
root
root sample 6; outgrowth in LB
+0.6
+0.2
-3.2
N.D.
+1.1
solid stress
Fraxetin 3 mM; solid stress
-0.3
+0.1
+0.9
N.D.
-2.0
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
-2.0
-0.0
+0.6
N.D.
+0.4
phage
P. simiae OR Antrim MOI 10
+0.2
+0.2
+1.0
N.D.
-2.4
agar plate interaction control
Taped volatile agar plate with no fungus
+0.6
-0.6
+0.9
N.D.
-1.8
carbon source
L-Valine (C)
+0.1
-0.1
-1.8
N.D.
+1.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-0.3
+0.3
+1.3
N.D.
+1.2
seeds
Growth on radish seeds for 72 hours
+0.6
+0.0
+3.8
N.D.
-0.9
seeds
Growth on radish seeds for 72 hours
-0.4
+0.1
+3.2
N.D.
+0.8
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