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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_09150 and PS417_09155 are separated by 2 nucleotides
PS417_09155 and PS417_09160 are separated by 190 nucleotides
PS417_09160 and PS417_09165 overlap by 4 nucleotides
PS417_09165 and PS417_09170 overlap by 4 nucleotides
PS417_09150: PS417_09150 - energy transducer TonB, at 1,989,784 to 1,990,512
_09150
PS417_09155: PS417_09155 - biopolymer transporter ExbD, at 1,990,515 to 1,990,922
_09155
PS417_09160: PS417_09160 - GNAT family acetyltransferase, at 1,991,113 to 1,991,667
_09160
PS417_09165: PS417_09165 - alkaline phosphatase, at 1,991,664 to 1,993,238
_09165
PS417_09170: PS417_09170 - alanyl-tRNA synthetase, at 1,993,235 to 1,993,861
_09170
Group
Condition
PS417
_09150
PS417
_09155
PS417
_09160
PS417
_09165
PS417
_09170
agar plate interaction control
Taped volatile agar plate with no fungus
-0.4
N.D.
-2.2
-0.4
-0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-2.2
N.D.
+0.0
+0.0
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.4
N.D.
-0.7
+0.0
-0.1
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
-0.5
N.D.
-1.6
+0.1
+0.0
carbon source
2'-Deoxyinosine 5 mM (C)
-0.9
N.D.
-1.0
+0.1
-0.0
supernatant
Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
-1.2
N.D.
+0.1
-0.5
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-2.0
N.D.
+0.3
+0.2
-0.0
phage
P. simiae OR Antrim MOI 10
+0.3
N.D.
-1.1
-0.5
-0.2
supernatant control
Vogels_fungal_media 0.1X
-2.0
N.D.
+0.6
+0.1
+0.1
liquid stress
Scopoletin 3 mM; liquid stress
-1.6
N.D.
+0.5
-0.0
+0.2
phage
P. simiae Grant Run MOI 0.1
-0.6
N.D.
-0.4
-0.5
+0.6
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-1.0
N.D.
+0.7
-0.1
-0.4
motility
inner cut, LB soft agar motility assay
-0.3
N.D.
-0.8
+0.3
+0.4
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.1
N.D.
+0.5
+0.5
+0.3
phage
JP1 MOI 10
+1.2
N.D.
-1.0
-0.1
+0.0
no phage control
Time6-nophage
-0.5
N.D.
+0.7
+0.3
-0.2
carbon source
L-Valine (C)
+0.5
N.D.
+0.6
-0.2
-0.6
supernatant
Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
+0.6
N.D.
+0.6
-0.4
-0.2
carbon source
Deoxyribonucleic from herring sperm 5 mg/mL (C)
+1.1
N.D.
-0.5
-0.2
+0.2
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+1.1
N.D.
-0.3
-0.3
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+1.0
N.D.
-0.4
+0.4
-0.2
soil
soil sample 3; outgrowth in LB
+1.4
N.D.
-0.3
-0.0
+0.1
phage
JP1 MOI 1
+0.8
N.D.
+0.8
-0.1
-0.3
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.4
N.D.
+1.7
+0.2
-0.1
phage
P. simiae OR1 MOI 0.1
+1.7
N.D.
+0.0
-0.3
-0.0
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+1.2
N.D.
+0.5
-0.2
-0.1
phage
JP1 MOI 10
+1.2
N.D.
+0.0
+0.7
-0.4
carbon source
2-Deoxy-D-Ribose 10 mM (C)
+0.9
N.D.
+0.6
-0.2
+0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+1.8
N.D.
+0.2
+0.0
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+1.4
N.D.
+1.0
-0.1
-0.1
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