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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_00080 and PS417_00085 are separated by 21 nucleotides
PS417_00085 and PS417_00090 overlap by 4 nucleotides
PS417_00090 and PS417_00095 are separated by 69 nucleotides
PS417_00095 and PS417_00100 are separated by 135 nucleotides
PS417_00080: PS417_00080 - potassium transporter peripheral membrane component, at 17,327 to 18,700
_00080
PS417_00085: PS417_00085 - 16S rRNA methyltransferase, at 18,722 to 20,032
_00085
PS417_00090: PS417_00090 - methionyl-tRNA formyltransferase, at 20,029 to 20,982
_00090
PS417_00095: PS417_00095 - peptide deformylase, at 21,052 to 21,558
_00095
PS417_00100: PS417_00100 - peptidoglycan-binding protein, at 21,694 to 22,470
_00100
Group
Condition
PS417
_00080
PS417
_00085
PS417
_00090
PS417
_00095
PS417
_00100
phage
JP1 MOI 10
-2.9
-1.2
N.D.
N.D.
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.7
-2.7
N.D.
N.D.
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.4
-2.6
N.D.
N.D.
-0.1
supernatant control
Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
-0.4
-2.0
N.D.
N.D.
-0.6
stress
Gentamicin 0.008 mg/ml
-2.6
-0.3
N.D.
N.D.
+0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.5
-2.2
N.D.
N.D.
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.4
-1.4
N.D.
N.D.
-1.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.9
-1.5
N.D.
N.D.
-0.4
agar plate interaction
Taped volatile agar plate with Trichoderma atroviridae IMI
-0.3
-2.0
N.D.
N.D.
-0.4
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.3
-1.9
N.D.
N.D.
-0.4
phage
P. simiae ORA MOI 10
-1.9
-0.3
N.D.
N.D.
-0.3
phage
P. simiae Grant Run MOI 10
-2.0
-0.3
N.D.
N.D.
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.8
-1.1
N.D.
N.D.
-0.6
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.4
-1.5
N.D.
N.D.
-0.5
phage
Wcs_1
-1.1
-1.3
N.D.
N.D.
-0.1
phage
P. simiae OR Antrim MOI 1
-1.1
-1.0
N.D.
N.D.
-0.3
phage
JP1 MOI 10
-0.9
-1.5
N.D.
N.D.
-0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.4
-2.3
N.D.
N.D.
+0.5
carbon source
L-Valine (C)
-1.4
-1.1
N.D.
N.D.
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.3
-1.8
N.D.
N.D.
-0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days
-0.5
-1.8
N.D.
N.D.
+0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-0.9
-1.1
N.D.
N.D.
+0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.8
-1.1
N.D.
N.D.
+0.3
phage
P. simiae ORA MOI 1
-1.4
-0.7
N.D.
N.D.
+0.7
phage
JP1 MOI 1
-1.3
+1.0
N.D.
N.D.
-0.6
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+0.7
-1.7
N.D.
N.D.
+0.4
phage
P. simiae ORA MOI 0.1
-2.0
+1.0
N.D.
N.D.
+0.4
carbon source
L-Valine (C)
-1.3
+1.2
N.D.
N.D.
-0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.8
+1.2
N.D.
N.D.
-0.3
stress
methylglyoxal 0.032 vol%
+3.0
+0.8
N.D.
N.D.
-0.4
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