Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_09055 and PS417_09060 are separated by 140 nucleotidesPS417_09060 and PS417_09065 are separated by 228 nucleotidesPS417_09065 and PS417_09070 are separated by 203 nucleotidesPS417_09070 and PS417_09075 are separated by 114 nucleotides PS417_09055: PS417_09055 - orotidine 5'-phosphate decarboxylase, at 1,971,048 to 1,971,746 _09055 PS417_09060: PS417_09060 - NADP-dependent oxidoreductase, at 1,971,887 to 1,972,894 _09060 PS417_09065: PS417_09065 - short-chain dehydrogenase, at 1,973,123 to 1,973,884 _09065 PS417_09070: PS417_09070 - GntR family transcriptional regulator, at 1,974,088 to 1,975,500 _09070 PS417_09075: PS417_09075 - chemotaxis protein, at 1,975,615 to 1,977,582 _09075
Group Condition PS417_09055 PS417_09060 PS417_09065 PS417_09070 PS417_09075
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.0 -0.3 -0.7 -0.2
phage P. simiae Grant Run MOI 0.1 N.D. -0.4 -0.8 -0.0 +0.3
phage JP1 MOI 0.1 N.D. +0.0 -0.6 +0.1 -0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.3 -0.5 +0.3 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.1 +0.2 -0.5 -0.6
root root sample 6; outgrowth in LB N.D. -0.1 -0.8 +0.3 -0.0
carbon source 2-Deoxy-D-ribonic lithium salt 10 mM (C) N.D. +0.1 -0.4 -0.6 +0.3
phage P. simiae Grant Run MOI 10 N.D. +0.3 -1.0 +0.3 -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.4 -0.5 -0.4 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Threonine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.3 -0.4 -0.3 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -0.4 -0.6 +0.4 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.7 -0.3 +0.3 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.0 -0.2 -0.5 +0.5
soil soil sample 5; outgrowth in LB N.D. -0.3 -0.7 +0.2 +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. -0.1 -0.7 +0.3 +0.3
no phage control no phage control N.D. +0.1 -0.6 +0.4 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.1 -0.7 +0.0 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.3 -0.3 +0.1 +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. +0.4 -0.4 +0.4 -0.1
rhizosphere rhizosphere sample 2; outgrowth in LB N.D. -0.0 -0.4 +0.4 +0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X N.D. -0.0 +0.4 +0.4 -0.4
seeds Growth on radish seeds for 24 hours N.D. +0.2 -0.5 +0.4 +0.3
rhizosphere rhizosphere sample 1; outgrowth in LB N.D. -0.3 +0.3 -0.1 +0.5
soil soil sample 4; outgrowth in LB N.D. -0.1 -0.2 +0.5 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.2 +0.5 +0.4 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.1 +0.1 +0.1 +0.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.2 +0.7 -0.0 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.2 +0.7 -0.1 +0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.2 +0.4 -0.1 +0.8
seeds Growth on radish seeds for 72 hours N.D. +0.0 +0.4 +0.5 +0.4
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