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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_08905 and PS417_08910 are separated by 204 nucleotides
PS417_08910 and PS417_08915 overlap by 1 nucleotides
PS417_08915 and PS417_08920 are separated by 416 nucleotides
PS417_08920 and PS417_08925 are separated by 167 nucleotides
PS417_08905: PS417_08905 - dihydrolipoamide dehydrogenase, at 1,938,848 to 1,940,284
_08905
PS417_08910: PS417_08910 - succinyl-CoA synthetase subunit beta, at 1,940,489 to 1,941,655
_08910
PS417_08915: PS417_08915 - succinyl-CoA synthetase subunit alpha, at 1,941,655 to 1,942,536
_08915
PS417_08920: PS417_08920 - branched-chain amino acid ABC transporter substrate-binding protein, at 1,942,953 to 1,944,266
_08920
PS417_08925: PS417_08925 - membrane protein, at 1,944,434 to 1,945,171
_08925
Group
Condition
PS417
_08905
PS417
_08910
PS417
_08915
PS417
_08920
PS417
_08925
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
N.D.
+0.2
-1.5
stress
methylglyoxal 0.032 vol%
N.D.
N.D.
N.D.
-0.5
-0.7
agar plate interaction control
Taped volatile agar plate with no fungus
N.D.
N.D.
N.D.
-0.5
-0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
N.D.
-0.4
-0.7
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
N.D.
N.D.
N.D.
-0.4
-0.6
motility
inner cut, LB soft agar motility assay
N.D.
N.D.
N.D.
-0.4
-0.6
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
N.D.
N.D.
-0.4
-0.6
motility
outer cut, LB soft agar motility assay
N.D.
N.D.
N.D.
-0.4
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
N.D.
N.D.
N.D.
-0.2
-0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
N.D.
N.D.
N.D.
-0.2
-0.6
in planta
Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
N.D.
N.D.
N.D.
-0.3
-0.6
soil
soil sample 7; outgrowth in LB
N.D.
N.D.
N.D.
-0.2
-0.7
nophagecontrol
Only library
N.D.
N.D.
N.D.
+0.1
-0.8
phage
P. simiae Grant Run MOI 10
N.D.
N.D.
N.D.
+0.1
-0.8
root
root sample 6; outgrowth in LB
N.D.
N.D.
N.D.
+0.5
-1.1
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
N.D.
N.D.
N.D.
+0.2
-0.8
soil
soil sample 2; outgrowth in LB
N.D.
N.D.
N.D.
-0.5
+0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
N.D.
N.D.
-0.8
+0.8
carbon source
L-Histidine (C)
N.D.
N.D.
N.D.
+0.6
-0.4
phage
Wcs_1
N.D.
N.D.
N.D.
-0.4
+0.6
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
N.D.
N.D.
-0.2
+0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
N.D.
-0.3
+0.8
carbon source
L-Valine (C)
N.D.
N.D.
N.D.
+0.9
-0.4
carbon source
L-Alanine (C)
N.D.
N.D.
N.D.
+0.9
+0.0
carbon source
2-Deoxy-D-Ribose 10 mM (C)
N.D.
N.D.
N.D.
+0.4
+0.6
carbon source
L-Alanine (C)
N.D.
N.D.
N.D.
+1.2
-0.1
carbon source
L-Valine (C)
N.D.
N.D.
N.D.
+1.0
+0.1
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
N.D.
N.D.
N.D.
+0.3
+0.9
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
N.D.
N.D.
N.D.
+1.0
+0.5
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
N.D.
N.D.
N.D.
+0.1
+1.5
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