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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_08850 and PS417_08855 are separated by 332 nucleotides
PS417_08855 and PS417_08860 are separated by 193 nucleotides
PS417_08860 and PS417_08865 overlap by 4 nucleotides
PS417_08865 and PS417_08870 are separated by 303 nucleotides
PS417_08850: PS417_08850 - beta (1-6) glucans synthase, at 1,924,299 to 1,925,852
_08850
PS417_08855: PS417_08855 - glycine/betaine ABC transporter substrate-binding protein, at 1,926,185 to 1,927,036
_08855
PS417_08860: PS417_08860 - small component of pyruvate transporter (yjcH-like)
(from data)
, at 1,927,230 to 1,927,541
_08860
PS417_08865: PS417_08865 - large component of pyruvate transporter (actP-like)
(from data)
, at 1,927,538 to 1,929,196
_08865
PS417_08870: PS417_08870 - type II citrate synthase, at 1,929,500 to 1,930,789
_08870
Group
Condition
PS417
_08850
PS417
_08855
PS417
_08860
PS417
_08865
PS417
_08870
carbon source
pyruvate (C)
-0.0
-0.1
-1.8
-1.6
N.D.
carbon source
pyruvate (C)
-0.2
+0.2
-1.8
-1.6
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.4
-0.3
-1.1
-0.1
N.D.
phage
P. simiae OR1 MOI 0.1
-0.6
-0.4
-0.5
-0.2
N.D.
carbon source
2'-Deoxyinosine 5 mM (C)
-0.1
-0.5
-0.7
-0.4
N.D.
nophagecontrol
Only library
-0.7
-0.6
-0.3
-0.0
N.D.
carbon source
D-Glucose-6-Phosphate (C)
-0.2
-0.4
-0.5
-0.4
N.D.
motility
outer cut, LB soft agar motility assay
-1.2
-0.4
+0.3
-0.1
N.D.
carbon source
Inosine (C)
-0.4
+0.1
-0.4
-0.6
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.2
-0.4
-1.1
+0.1
N.D.
motility
outer cut, LB soft agar motility assay
-0.6
-0.8
+0.1
+0.0
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
+0.1
-1.0
-0.3
N.D.
rhizosphere
rhizosphere sample 2; outgrowth in LB
-0.6
+0.1
-0.6
+0.2
N.D.
stress
methylglyoxal 0.032 vol%
+0.2
-0.4
-0.9
+0.2
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.4
-0.5
-0.4
-0.2
N.D.
seeds
Growth on radish seeds for 72 hours
+0.3
-0.8
+0.2
-0.3
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.3
+0.1
-1.0
+0.1
N.D.
phage
Wcs_1
+0.6
-0.3
-0.6
-0.0
N.D.
soil
soil sample 2; outgrowth in LB
-0.6
+0.3
+0.8
-0.2
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
+0.5
+0.4
-0.4
-0.3
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.3
-0.5
+0.7
+0.0
N.D.
soil
soil sample 7; outgrowth in LB
-0.6
+0.2
+0.5
+0.3
N.D.
supernatant
Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
+0.2
+0.4
-0.5
+0.5
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.2
+0.7
+0.1
-0.4
N.D.
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+0.9
-0.4
+0.2
+0.1
N.D.
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+0.7
+0.4
-0.2
+0.2
N.D.
carbon source
Gly-Glu (C)
-0.1
-0.0
+0.9
+0.4
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.3
+0.0
+0.9
+0.2
N.D.
soil
soil sample 4; outgrowth in LB
-0.1
+0.7
+1.0
-0.1
N.D.
soil
soil sample 3; outgrowth in LB
+0.6
+0.8
+0.5
-0.0
N.D.
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