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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_08390 and PS417_08395 are separated by 45 nucleotides
PS417_08395 and PS417_08400 overlap by 1 nucleotides
PS417_08400 and PS417_08405 are separated by 10 nucleotides
PS417_08405 and PS417_08410 are separated by 58 nucleotides
PS417_08390: PS417_08390 - transcription elongation factor GreB, at 1,839,603 to 1,840,076
_08390
PS417_08395: PS417_08395 - ABC transporter permease, at 1,840,122 to 1,842,632
_08395
PS417_08400: PS417_08400 - ABC transporter, at 1,842,632 to 1,843,315
_08400
PS417_08405: PS417_08405 - esterase, at 1,843,326 to 1,843,931
_08405
PS417_08410: PS417_08410 - hypothetical protein, at 1,843,990 to 1,844,283
_08410
Group
Condition
PS417
_08390
PS417
_08395
PS417
_08400
PS417
_08405
PS417
_08410
phage
P. simiae Grant Run MOI 0.1
+0.1
+0.0
N.D.
+0.0
-3.8
liquid stress
Scopoletin 3 mM; liquid stress
-0.0
+0.2
N.D.
-0.1
-3.7
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.5
-0.3
N.D.
-0.6
-2.1
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB
+0.1
-0.1
N.D.
+0.1
-3.4
soil
soil sample 4; outgrowth in LB
-0.2
-0.2
N.D.
-0.4
-2.5
soil
soil sample 5; outgrowth in LB
-0.6
-0.3
N.D.
+0.2
-2.4
solid stress
Fraxetin 3 mM; solid stress
-0.0
-0.3
N.D.
-0.3
-2.2
liquid stress
80% Methanol 2 mM; liquid stress
-0.0
+0.0
N.D.
+0.1
-2.9
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.8X; growth supplemented with 0.4X LB
-0.1
-0.4
N.D.
-0.0
-2.2
nophagecontrol
Only library
-0.3
-0.1
N.D.
-0.0
-2.3
motility
inner cut, LB soft agar motility assay
+0.3
+0.1
N.D.
-0.3
-2.7
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.1
-0.0
N.D.
-0.2
-2.5
solid stress
Scopoletin 2 mM; solid stress
-0.1
-0.1
N.D.
-0.2
-2.1
liquid stress
Scopoletin 1 mM; liquid stress
-0.2
-0.1
N.D.
-0.0
-2.2
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
-0.2
-0.3
N.D.
+0.1
-2.1
agar plate interaction
Taped volatile agar plate with Trichoderma atroviridae IMI
-0.3
-0.2
N.D.
-0.2
-1.7
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.4
+0.0
N.D.
-0.0
-1.9
motility
outer cut, LB soft agar motility assay
-0.3
+0.1
N.D.
-0.2
-2.0
motility
outer cut, LB soft agar motility assay
+0.1
-0.0
N.D.
-0.2
-2.2
seeds
Growth on radish seeds for 72 hours
+0.2
-0.1
N.D.
-0.1
-2.2
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB
+0.2
+0.0
N.D.
-0.0
-2.4
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X
+0.0
-0.2
N.D.
+0.0
-1.9
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.1
-0.3
N.D.
-0.3
-1.6
supernatant control
Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
+0.2
-0.3
N.D.
-0.1
-1.7
stress
Paraquat dichloride 0.008 mg/ml
+0.0
+0.2
N.D.
-0.1
-2.1
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.5
-0.3
N.D.
+0.1
-2.2
stress
Paraquat dichloride 0.002 mg/ml
-0.4
+0.3
N.D.
+0.1
-1.8
agar plate interaction control
Taped volatile agar plate with no fungus
+0.2
+0.5
N.D.
-0.0
-1.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
+0.6
N.D.
+0.3
-1.0
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.3
-0.1
N.D.
-0.1
+1.8
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