Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_08390 and PS417_08395 are separated by 45 nucleotidesPS417_08395 and PS417_08400 overlap by 1 nucleotidesPS417_08400 and PS417_08405 are separated by 10 nucleotidesPS417_08405 and PS417_08410 are separated by 58 nucleotides PS417_08390: PS417_08390 - transcription elongation factor GreB, at 1,839,603 to 1,840,076 _08390 PS417_08395: PS417_08395 - ABC transporter permease, at 1,840,122 to 1,842,632 _08395 PS417_08400: PS417_08400 - ABC transporter, at 1,842,632 to 1,843,315 _08400 PS417_08405: PS417_08405 - esterase, at 1,843,326 to 1,843,931 _08405 PS417_08410: PS417_08410 - hypothetical protein, at 1,843,990 to 1,844,283 _08410
Group Condition PS417_08390 PS417_08395 PS417_08400 PS417_08405 PS417_08410
phage P. simiae Grant Run MOI 0.1 +0.1 +0.0 N.D. +0.0 -3.8
liquid stress Scopoletin 3 mM; liquid stress -0.0 +0.2 N.D. -0.1 -3.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.5 -0.3 N.D. -0.6 -2.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB +0.1 -0.1 N.D. +0.1 -3.4
soil soil sample 4; outgrowth in LB -0.2 -0.2 N.D. -0.4 -2.5
soil soil sample 5; outgrowth in LB -0.6 -0.3 N.D. +0.2 -2.4
solid stress Fraxetin 3 mM; solid stress -0.0 -0.3 N.D. -0.3 -2.2
liquid stress 80% Methanol 2 mM; liquid stress -0.0 +0.0 N.D. +0.1 -2.9
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.8X; growth supplemented with 0.4X LB -0.1 -0.4 N.D. -0.0 -2.2
nophagecontrol Only library -0.3 -0.1 N.D. -0.0 -2.3
motility inner cut, LB soft agar motility assay +0.3 +0.1 N.D. -0.3 -2.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 -0.0 N.D. -0.2 -2.5
solid stress Scopoletin 2 mM; solid stress -0.1 -0.1 N.D. -0.2 -2.1
liquid stress Scopoletin 1 mM; liquid stress -0.2 -0.1 N.D. -0.0 -2.2
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x -0.2 -0.3 N.D. +0.1 -2.1
agar plate interaction Taped volatile agar plate with Trichoderma atroviridae IMI -0.3 -0.2 N.D. -0.2 -1.7
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.4 +0.0 N.D. -0.0 -1.9
motility outer cut, LB soft agar motility assay -0.3 +0.1 N.D. -0.2 -2.0
motility outer cut, LB soft agar motility assay +0.1 -0.0 N.D. -0.2 -2.2
seeds Growth on radish seeds for 72 hours +0.2 -0.1 N.D. -0.1 -2.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.2 +0.0 N.D. -0.0 -2.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X +0.0 -0.2 N.D. +0.0 -1.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 -0.3 N.D. -0.3 -1.6
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.2 -0.3 N.D. -0.1 -1.7
stress Paraquat dichloride 0.008 mg/ml +0.0 +0.2 N.D. -0.1 -2.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 -0.3 N.D. +0.1 -2.2
stress Paraquat dichloride 0.002 mg/ml -0.4 +0.3 N.D. +0.1 -1.8
agar plate interaction control Taped volatile agar plate with no fungus +0.2 +0.5 N.D. -0.0 -1.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 +0.6 N.D. +0.3 -1.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.3 -0.1 N.D. -0.1 +1.8
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