Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_08300 and PS417_08305 are separated by 37 nucleotidesPS417_08305 and PS417_08310 are separated by 131 nucleotidesPS417_08310 and PS417_08315 are separated by 216 nucleotidesPS417_08315 and PS417_08320 are separated by 44 nucleotides PS417_08300: PS417_08300 - histidine kinase, at 1,817,236 to 1,819,887 _08300 PS417_08305: PS417_08305 - transcriptional regulator, at 1,819,925 to 1,820,614 _08305 PS417_08310: PS417_08310 - alpha/beta hydrolase, at 1,820,746 to 1,821,783 _08310 PS417_08315: PS417_08315 - hypothetical protein, at 1,822,000 to 1,822,278 _08315 PS417_08320: PS417_08320 - metal-chelation protein CHAD, at 1,822,323 to 1,823,084 _08320
Group Condition PS417_08300 PS417_08305 PS417_08310 PS417_08315 PS417_08320
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 +0.2 -0.0 -0.2 -3.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 -0.4 -0.2 -0.3 -1.5
carbon source 2'-Deoxyinosine 5 mM (C) -0.3 -0.3 -0.4 -0.4 -0.6
phage P. simiae OR Antrim MOI 0.1 -0.0 -0.2 -0.7 +0.3 -1.3
solid stress Fraxetin 3 mM; solid stress +0.1 +0.2 -2.5 +0.4 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 -0.3 -0.1 +0.6 -1.8
motility inner cut, LB soft agar motility assay -0.0 -0.2 +0.1 -0.2 -1.5
solid stress Fraxetin 3 mM; solid stress -0.1 -0.0 -1.1 +0.4 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 +0.3 +0.4 +0.0 -2.1
phage P. simiae OR Antrim MOI 10 -0.2 +0.5 -0.3 +0.4 -1.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 -0.1 +0.3 +0.6 -1.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 +0.8 -0.4 -0.4 -0.4
solid stress Fraxetin 3 mM; solid stress +0.1 +0.5 -1.8 +0.7 +0.1
phage P. simiae ORA MOI 10 -0.2 -0.8 +0.5 +0.4 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.5 +0.0 -0.2 -0.4 +1.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -0.0 +0.3 -0.3 -0.8 +0.8
phage JP1 MOI 10 +0.1 -0.3 +0.3 +0.7 -0.8
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x +0.3 +0.4 +0.6 -0.3 -0.9
carbon source L-Valine (C) +0.1 -0.2 -0.7 -0.2 +1.1
agar plate interaction Taped volatile agar plate with Trichoderma atroviridae IMI -0.2 -0.3 -0.5 -0.3 +1.4
seeds Growth on radish seeds for 72 hours -0.3 -0.4 -0.1 +0.0 +1.2
soil soil sample 4; outgrowth in LB +0.2 +0.5 -0.5 +1.0 -0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.4 -0.3 +0.4 +0.5 +0.9
seeds Growth on radish seeds for 72 hours -0.2 -0.1 -0.8 +0.3 +1.9
agar plate interaction control Taped volatile agar plate with no fungus +0.1 -0.1 +0.6 -0.4 +1.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 +0.5 -0.1 +0.6 +0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.5 -0.4 +0.8 +0.2 +0.6
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.4 +0.2 +0.6 -0.1 +0.7
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB +0.2 +0.2 +0.3 -0.1 +1.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.1 +0.2 +0.3 +0.1 +1.5
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