Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_07975 and PS417_07980 are separated by 71 nucleotidesPS417_07980 and PS417_07985 overlap by 4 nucleotidesPS417_07985 and PS417_07990 are separated by 154 nucleotidesPS417_07990 and PS417_07995 are separated by 1 nucleotides PS417_07975: PS417_07975 - hypothetical protein, at 1,746,682 to 1,747,056 _07975 PS417_07980: PS417_07980 - twitching motility protein PilT, at 1,747,128 to 1,747,523 _07980 PS417_07985: PS417_07985 - AbrB family transcriptional regulator, at 1,747,520 to 1,747,777 _07985 PS417_07990: PS417_07990 - endoribonuclease L-PSP, at 1,747,932 to 1,748,324 _07990 PS417_07995: PS417_07995 - LOG family protein, at 1,748,326 to 1,749,699 _07995
Group Condition PS417_07975 PS417_07980 PS417_07985 PS417_07990 PS417_07995
agar plate interaction control Taped volatile agar plate with no fungus N.D. -0.4 -1.4 -0.4 -0.2
carbon source Ethanol (C) N.D. -0.0 -1.3 -0.1 -0.8
soil soil sample 3; outgrowth in LB N.D. +0.1 -1.8 -0.2 -0.2
carbon source L-Valine (C) N.D. +0.2 -0.7 -0.5 -1.0
soil soil sample 8; outgrowth in LB N.D. -0.2 -1.6 +0.3 -0.4
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. -0.3 -1.7 +0.5 -0.0
carbon source Carbon source L-Citrulline 5 mM N.D. -0.6 -0.2 +0.4 -0.8
carbon source L-Valine (C) N.D. +0.3 +0.2 -0.5 -1.3
stress 1-ethyl-3-methylimidazolium acetate 35 mM N.D. +0.2 -0.8 +0.2 -0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. +0.2 -1.2 -0.5 +0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.2 -1.1 +0.2 -0.4
phage P. simiae OR Antrim MOI 0.1 N.D. +0.3 -1.3 -0.3 +0.5
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x N.D. +0.1 -0.1 +0.5 -1.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.3 -0.7 +0.8 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.5 -1.1 +0.1 +0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.8 +0.5 +0.3 -0.2
stress Doxycycline hyclate 0.001 mg/ml N.D. +0.4 -0.9 +0.6 +0.1
carbon source 2'-Deoxyinosine 5 mM (C) N.D. -0.0 +0.7 +0.7 -0.9
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -0.5 +0.6 -0.2 +0.6
phage JP1 MOI 1 N.D. -0.4 +1.3 -0.2 -0.0
stress methylglyoxal 0.032 vol% N.D. +0.4 +0.5 -0.6 +0.4
carbon source 2'-Deoxyinosine 5 mM (C) N.D. +0.2 +0.9 +0.4 -0.8
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +1.0 +0.3 -0.4 +0.3
phage JP1 MOI 0.1 N.D. -0.4 +1.4 +0.6 -0.5
solid stress 80% Methanol 1 mM; solid stress N.D. +0.5 +1.0 -0.3 +0.2
motility inner cut, LB soft agar motility assay N.D. -0.2 +1.5 +0.0 +0.2
seeds Growth on radish seeds for 24 hours N.D. +0.3 +0.8 +0.7 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.1 +1.3 +0.3 +0.4
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB N.D. +0.6 +0.8 +0.6 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.2 +4.0 +0.3 +0.3
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