Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_07965 and PS417_07970 are separated by 94 nucleotidesPS417_07970 and PS417_07975 are separated by 6 nucleotidesPS417_07975 and PS417_07980 are separated by 71 nucleotidesPS417_07980 and PS417_07985 overlap by 4 nucleotides PS417_07965: PS417_07965 - multidrug DMT transporter permease, at 1,745,316 to 1,746,215 _07965 PS417_07970: PS417_07970 - hypothetical protein, at 1,746,310 to 1,746,675 _07970 PS417_07975: PS417_07975 - hypothetical protein, at 1,746,682 to 1,747,056 _07975 PS417_07980: PS417_07980 - twitching motility protein PilT, at 1,747,128 to 1,747,523 _07980 PS417_07985: PS417_07985 - AbrB family transcriptional regulator, at 1,747,520 to 1,747,777 _07985
Group Condition PS417_07965 PS417_07970 PS417_07975 PS417_07980 PS417_07985
agar plate interaction control Taped volatile agar plate with no fungus -0.0 -0.1 N.D. -0.4 -1.4
soil soil sample 8; outgrowth in LB -0.0 -0.0 N.D. -0.2 -1.6
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.1 +0.1 N.D. -0.3 -1.7
soil soil sample 3; outgrowth in LB +0.1 +0.0 N.D. +0.1 -1.8
phage P. simiae ORA MOI 10 -0.3 -0.2 N.D. -0.0 -1.1
carbon source Ethanol (C) -0.0 -0.1 N.D. -0.0 -1.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.0 -0.2 N.D. +0.1 -1.4
stress Chloramphenicol 0.0075 mg/ml -0.1 -0.1 N.D. +0.1 -1.2
phage P. simiae OR Antrim MOI 0.1 -0.2 +0.1 N.D. +0.3 -1.3
phage P. simiae ORA MOI 0.1 -0.0 -0.2 N.D. +0.2 -1.1
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x +0.2 +0.0 N.D. -0.6 -0.7
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.1 +0.3 N.D. -0.6 -0.8
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.2 -0.1 N.D. +0.2 -1.2
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.4 -0.2 N.D. -0.5 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.0 +0.1 N.D. +0.5 -1.1
stress Doxycycline hyclate 0.001 mg/ml -0.1 +0.1 N.D. +0.4 -0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 -0.2 N.D. -0.8 +0.5
phage P. simiae Grant Run MOI 0.1 -0.1 -0.1 N.D. -0.4 +0.8
no phage control Time6-nophage -0.2 +0.0 N.D. -0.3 +1.1
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 -0.1 N.D. +1.0 +0.3
phage JP1 MOI 1 +0.1 -0.0 N.D. -0.4 +1.3
seeds Growth on radish seeds for 24 hours +0.1 -0.3 N.D. +0.3 +0.8
phage JP1 MOI 0.1 +0.5 -0.0 N.D. -0.4 +1.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 -0.0 N.D. -0.0 +1.1
stress Benzalkonium Chloride 0.012 mg/ml +0.2 -0.0 N.D. +0.1 +1.1
solid stress 80% Methanol 1 mM; solid stress +0.1 -0.0 N.D. +0.5 +1.0
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB +0.2 -0.0 N.D. +0.6 +0.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 -0.1 N.D. +0.1 +1.3
motility inner cut, LB soft agar motility assay +0.2 +0.1 N.D. -0.2 +1.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.2 +0.3 N.D. +0.2 +4.0
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