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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_07965 and PS417_07970 are separated by 94 nucleotides
PS417_07970 and PS417_07975 are separated by 6 nucleotides
PS417_07975 and PS417_07980 are separated by 71 nucleotides
PS417_07980 and PS417_07985 overlap by 4 nucleotides
PS417_07965: PS417_07965 - multidrug DMT transporter permease, at 1,745,316 to 1,746,215
_07965
PS417_07970: PS417_07970 - hypothetical protein, at 1,746,310 to 1,746,675
_07970
PS417_07975: PS417_07975 - hypothetical protein, at 1,746,682 to 1,747,056
_07975
PS417_07980: PS417_07980 - twitching motility protein PilT, at 1,747,128 to 1,747,523
_07980
PS417_07985: PS417_07985 - AbrB family transcriptional regulator, at 1,747,520 to 1,747,777
_07985
Group
Condition
PS417
_07965
PS417
_07970
PS417
_07975
PS417
_07980
PS417
_07985
agar plate interaction control
Taped volatile agar plate with no fungus
-0.0
-0.1
N.D.
-0.4
-1.4
soil
soil sample 8; outgrowth in LB
-0.0
-0.0
N.D.
-0.2
-1.6
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+0.1
+0.1
N.D.
-0.3
-1.7
soil
soil sample 3; outgrowth in LB
+0.1
+0.0
N.D.
+0.1
-1.8
phage
P. simiae ORA MOI 10
-0.3
-0.2
N.D.
-0.0
-1.1
carbon source
Ethanol (C)
-0.0
-0.1
N.D.
-0.0
-1.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.0
-0.2
N.D.
+0.1
-1.4
stress
Chloramphenicol 0.0075 mg/ml
-0.1
-0.1
N.D.
+0.1
-1.2
phage
P. simiae OR Antrim MOI 0.1
-0.2
+0.1
N.D.
+0.3
-1.3
phage
P. simiae ORA MOI 0.1
-0.0
-0.2
N.D.
+0.2
-1.1
supernatant
RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x
+0.2
+0.0
N.D.
-0.6
-0.7
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
+0.1
+0.3
N.D.
-0.6
-0.8
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.2
-0.1
N.D.
+0.2
-1.2
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.4
-0.2
N.D.
-0.5
+0.6
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
+0.0
+0.1
N.D.
+0.5
-1.1
stress
Doxycycline hyclate 0.001 mg/ml
-0.1
+0.1
N.D.
+0.4
-0.9
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
-0.2
N.D.
-0.8
+0.5
phage
P. simiae Grant Run MOI 0.1
-0.1
-0.1
N.D.
-0.4
+0.8
no phage control
Time6-nophage
-0.2
+0.0
N.D.
-0.3
+1.1
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.2
-0.1
N.D.
+1.0
+0.3
phage
JP1 MOI 1
+0.1
-0.0
N.D.
-0.4
+1.3
seeds
Growth on radish seeds for 24 hours
+0.1
-0.3
N.D.
+0.3
+0.8
phage
JP1 MOI 0.1
+0.5
-0.0
N.D.
-0.4
+1.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
-0.0
N.D.
-0.0
+1.1
stress
Benzalkonium Chloride 0.012 mg/ml
+0.2
-0.0
N.D.
+0.1
+1.1
solid stress
80% Methanol 1 mM; solid stress
+0.1
-0.0
N.D.
+0.5
+1.0
supernatant control
Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
+0.2
-0.0
N.D.
+0.6
+0.8
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
-0.1
N.D.
+0.1
+1.3
motility
inner cut, LB soft agar motility assay
+0.2
+0.1
N.D.
-0.2
+1.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
+0.2
+0.3
N.D.
+0.2
+4.0
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