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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_00750 and PS417_00755 are separated by 2 nucleotides
PS417_00755 and PS417_00760 are separated by 367 nucleotides
PS417_00760 and PS417_00765 are separated by 31 nucleotides
PS417_00765 and PS417_00770 are separated by 904 nucleotides
PS417_00750: PS417_00750 - N-glycosyltransferase, at 159,282 to 160,622
_00750
PS417_00755: PS417_00755 - biofilm PGA synthesis protein PgaD, at 160,625 to 161,107
_00755
PS417_00760: PS417_00760 - ShlB family hemolysin secretion/activation protein, at 161,475 to 163,196
_00760
PS417_00765: PS417_00765 - filamentous hemagglutinin, at 163,228 to 170,496
_00765
PS417_00770: PS417_00770 - autotransporter, at 171,401 to 173,989
_00770
Group
Condition
PS417
_00750
PS417
_00755
PS417
_00760
PS417
_00765
PS417
_00770
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.3
-4.2
-0.0
-0.1
-0.0
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.0
-4.2
-0.2
-0.2
+0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.7
-4.1
+0.0
-0.0
+0.4
motility
outer cut, LB soft agar motility assay
-0.1
-4.3
+0.0
-0.0
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
-4.2
+0.1
-0.0
+0.1
phage
P. simiae ORA MOI 0.1
-0.3
-3.8
-0.1
-0.0
+0.2
solid stress
Fraxetin 2 mM; solid stress
-0.7
-3.7
+0.2
+0.0
+0.1
solid stress
Fraxetin 3 mM; solid stress
-0.3
-3.6
-0.1
+0.1
-0.1
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X
-0.3
-3.6
-0.0
-0.0
-0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
-4.0
+0.1
-0.0
+0.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.2
-3.3
+0.0
-0.0
-0.1
stress
Phosphomycin 0.002 mg/ml
-0.2
-3.3
-0.0
-0.1
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Aspartic Acid; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.0
-3.5
-0.1
+0.1
+0.0
supernatant control
Vogels_fungal_media 0.1X
-0.1
-3.1
-0.1
-0.1
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
-4.1
+0.3
+0.1
+0.0
phage
P. simiae Grant Run MOI 0.1
+0.1
-3.6
+0.0
-0.1
+0.1
phage
JP1 MOI 0.1
+0.2
-3.2
-0.1
-0.1
-0.0
stress
Phosphomycin 0.003 mg/ml
+0.2
-3.2
+0.1
-0.1
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
-4.1
+0.4
+0.0
+0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.1
-3.2
+0.2
+0.2
-0.0
phage
P. simiae OR Antrim MOI 1
-0.3
-2.2
+0.3
-0.2
-0.0
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.8
-3.1
+0.1
+0.1
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.3
-2.2
+0.3
+0.0
-0.1
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.1
+2.4
-0.1
-0.2
-0.3
seeds
Growth on radish seeds for 72 hours
+0.0
+2.4
-0.2
-0.1
+0.1
liquid stress
Fraxetin 3 mM; liquid stress
+1.2
+1.3
+0.1
-0.1
+0.2
carbon source
L-Valine (C)
+0.8
+1.4
-0.1
+0.3
+0.3
liquid stress
Fraxetin 2 mM; liquid stress
+1.7
+1.9
+0.1
-0.0
+0.1
liquid stress
80% Methanol 3 mM; liquid stress
+1.7
+2.3
+0.0
+0.0
+0.0
in planta
Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.4
+3.3
+0.2
-0.0
+0.4
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