Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_00065 and PS417_00070 are separated by 40 nucleotidesPS417_00070 and PS417_00075 are separated by 136 nucleotidesPS417_00075 and PS417_00080 are separated by 17 nucleotidesPS417_00080 and PS417_00085 are separated by 21 nucleotides PS417_00065: PS417_00065 - 3-methyladenine DNA glycosylase, at 15,376 to 15,933 _00065 PS417_00070: PS417_00070 - lipid A biosynthesis lauroyl acyltransferase, at 15,974 to 16,861 _00070 PS417_00075: PS417_00075 - hypothetical protein, at 16,998 to 17,309 _00075 PS417_00080: PS417_00080 - potassium transporter peripheral membrane component, at 17,327 to 18,700 _00080 PS417_00085: PS417_00085 - 16S rRNA methyltransferase, at 18,722 to 20,032 _00085
Group Condition PS417_00065 PS417_00070 PS417_00075 PS417_00080 PS417_00085
phage JP1 MOI 10 -2.7 N.D. +0.1 -2.9 -1.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.2 N.D. -1.1 -0.7 -2.7
phage P. simiae Grant Run MOI 10 -1.5 N.D. -0.6 -2.0 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.0 N.D. -2.0 -0.7 -0.7
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB -2.0 N.D. +0.1 -0.4 -2.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.1 N.D. -1.6 -1.1 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.3 N.D. -0.6 -0.8 -1.1
phage P. simiae OR Antrim MOI 1 -0.4 N.D. -1.2 -1.1 -1.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.7 N.D. -1.3 -0.8 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.6 N.D. -1.6 -0.9 -1.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -2.0 N.D. -0.7 -0.5 -0.0
phage P. simiae OR Antrim MOI 10 -1.5 N.D. -0.4 -1.3 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 N.D. -0.4 -0.4 -2.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +1.1 N.D. -0.8 -0.4 -2.6
phage JP1 MOI 0.1 -0.9 N.D. -1.0 -1.1 +0.2
nophagecontrol Only library -0.6 N.D. -1.0 +0.3 -1.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 N.D. -0.5 -0.5 -2.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 N.D. -1.0 -0.8 -1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.3 N.D. +0.5 -0.5 -1.3
stress Thallium(I) acetate 0.0625 mg/ml -0.2 N.D. -2.0 -0.9 +0.5
stress Gentamicin 0.008 mg/ml -0.1 N.D. +0.4 -2.6 -0.3
solid stress Fraxetin 3 mM; solid stress +0.2 N.D. -1.3 -1.4 +0.3
stress Paraquat dichloride 0.002 mg/ml -0.1 N.D. -1.5 -1.0 +0.7
phage P. simiae ORA MOI 0.1 +0.0 N.D. -0.4 -2.0 +1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 N.D. +1.3 -0.3 -1.8
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.3 N.D. -0.4 -0.1 -1.5
phage JP1 MOI 1 -0.9 N.D. +0.7 -1.3 +1.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.7 N.D. -0.8 -0.5 -0.4
carbon source L-Valine (C) +1.6 N.D. +1.1 -1.4 -1.1
stress methylglyoxal 0.032 vol% -0.5 N.D. -1.1 +3.0 +0.8
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