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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_00065 and PS417_00070 are separated by 40 nucleotides
PS417_00070 and PS417_00075 are separated by 136 nucleotides
PS417_00075 and PS417_00080 are separated by 17 nucleotides
PS417_00080 and PS417_00085 are separated by 21 nucleotides
PS417_00065: PS417_00065 - 3-methyladenine DNA glycosylase, at 15,376 to 15,933
_00065
PS417_00070: PS417_00070 - lipid A biosynthesis lauroyl acyltransferase, at 15,974 to 16,861
_00070
PS417_00075: PS417_00075 - hypothetical protein, at 16,998 to 17,309
_00075
PS417_00080: PS417_00080 - potassium transporter peripheral membrane component, at 17,327 to 18,700
_00080
PS417_00085: PS417_00085 - 16S rRNA methyltransferase, at 18,722 to 20,032
_00085
Group
Condition
PS417
_00065
PS417
_00070
PS417
_00075
PS417
_00080
PS417
_00085
phage
JP1 MOI 10
-2.7
N.D.
+0.1
-2.9
-1.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.2
N.D.
-1.1
-0.7
-2.7
phage
P. simiae Grant Run MOI 10
-1.5
N.D.
-0.6
-2.0
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.0
N.D.
-2.0
-0.7
-0.7
supernatant control
Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
-2.0
N.D.
+0.1
-0.4
-2.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.1
N.D.
-1.6
-1.1
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.3
N.D.
-0.6
-0.8
-1.1
phage
P. simiae OR Antrim MOI 1
-0.4
N.D.
-1.2
-1.1
-1.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.7
N.D.
-1.3
-0.8
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.6
N.D.
-1.6
-0.9
-1.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-2.0
N.D.
-0.7
-0.5
-0.0
phage
P. simiae OR Antrim MOI 10
-1.5
N.D.
-0.4
-1.3
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
N.D.
-0.4
-0.4
-2.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+1.1
N.D.
-0.8
-0.4
-2.6
phage
JP1 MOI 0.1
-0.9
N.D.
-1.0
-1.1
+0.2
nophagecontrol
Only library
-0.6
N.D.
-1.0
+0.3
-1.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
N.D.
-0.5
-0.5
-2.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
N.D.
-1.0
-0.8
-1.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-1.3
N.D.
+0.5
-0.5
-1.3
stress
Thallium(I) acetate 0.0625 mg/ml
-0.2
N.D.
-2.0
-0.9
+0.5
stress
Gentamicin 0.008 mg/ml
-0.1
N.D.
+0.4
-2.6
-0.3
solid stress
Fraxetin 3 mM; solid stress
+0.2
N.D.
-1.3
-1.4
+0.3
stress
Paraquat dichloride 0.002 mg/ml
-0.1
N.D.
-1.5
-1.0
+0.7
phage
P. simiae ORA MOI 0.1
+0.0
N.D.
-0.4
-2.0
+1.0
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.2
N.D.
+1.3
-0.3
-1.8
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+1.3
N.D.
-0.4
-0.1
-1.5
phage
JP1 MOI 1
-0.9
N.D.
+0.7
-1.3
+1.0
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+1.7
N.D.
-0.8
-0.5
-0.4
carbon source
L-Valine (C)
+1.6
N.D.
+1.1
-1.4
-1.1
stress
methylglyoxal 0.032 vol%
-0.5
N.D.
-1.1
+3.0
+0.8
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