Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_07610 and PS417_07615 are separated by 263 nucleotidesPS417_07615 and PS417_07620 are separated by 136 nucleotidesPS417_07620 and PS417_07625 are separated by 49 nucleotidesPS417_07625 and PS417_07630 are separated by 9 nucleotides PS417_07610: PS417_07610 - LexA family transcriptional regulator, at 1,674,577 to 1,675,185 _07610 PS417_07615: PS417_07615 - TetR family transcriptional regulator, at 1,675,449 to 1,676,156 _07615 PS417_07620: PS417_07620 - beta-hexosaminidase, at 1,676,293 to 1,677,303 _07620 PS417_07625: PS417_07625 - helicase, at 1,677,353 to 1,680,046 _07625 PS417_07630: PS417_07630 - hypothetical protein, at 1,680,056 to 1,680,622 _07630
Group Condition PS417_07610 PS417_07615 PS417_07620 PS417_07625 PS417_07630
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -0.6 -3.4 -0.2 -0.7
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -0.7 -3.5 -0.0 -0.2
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x N.D. -2.6 -1.8 +0.0 -0.0
liquid stress Fraxetin 3 mM; liquid stress N.D. -4.0 -0.0 -0.1 -0.1
soil soil sample 6; outgrowth in LB N.D. -0.2 -4.1 -0.1 -0.0
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -0.9 -3.2 +0.1 -0.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -0.9 -3.5 +0.2 +0.1
seeds Growth on radish seeds for 72 hours N.D. -0.8 -2.9 -0.1 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -2.1 -1.7 +0.1 -0.2
liquid stress Fraxetin 3 mM; liquid stress N.D. -3.5 -0.1 -0.2 -0.0
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -1.7 -2.2 -0.0 +0.2
stress Polymyxin B sulfate 0.006 mg/ml N.D. +0.1 -3.7 -0.1 +0.0
stress Gentamicin 0.008 mg/ml N.D. -0.3 -2.8 -0.4 +0.0
motility outer cut, LB soft agar motility assay N.D. -0.6 -2.7 -0.2 -0.0
motility outer cut, LB soft agar motility assay N.D. -0.5 -3.6 +0.1 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.8 -2.8 -0.1 +0.3
carbon source 2-Deoxy-D-Ribose (C) N.D. -2.9 -0.9 +0.0 +0.3
soil soil sample 2; outgrowth in LB N.D. +0.1 -3.3 -0.3 +0.1
stress Phosphomycin 0.003 mg/ml N.D. -0.0 -3.3 -0.1 +0.1
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. +0.1 -2.9 -0.2 +0.4
solid stress Fraxetin 3 mM; solid stress N.D. -2.7 +0.9 -0.3 -0.3
rhizosphere rhizosphere sample 1; outgrowth in LB N.D. +1.1 -3.1 -0.2 +0.2
root root sample 6; outgrowth in LB N.D. +0.9 -2.3 +0.1 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +2.2 -1.3 +0.0 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +2.1 -1.3 +0.1 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +2.8 -0.8 -0.0 -0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days N.D. +2.5 -1.3 +0.2 +0.7
stress R2A with Fusaric 120 ug/mL N.D. +1.7 +1.2 -0.4 -0.4
stress R2A with Fusaric 120 ug/mL N.D. +1.9 +1.4 -0.2 -0.3
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. +4.0 -0.9 +0.1 -0.3
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