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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_07520 and PS417_07525 are separated by 134 nucleotides
PS417_07525 and PS417_07530 are separated by 13 nucleotides
PS417_07530 and PS417_07535 are separated by 140 nucleotides
PS417_07535 and PS417_07540 are separated by 53 nucleotides
PS417_07520: PS417_07520 - 23S rRNA methyltransferase, at 1,654,257 to 1,655,333
_07520
PS417_07525: PS417_07525 - sulfurtransferase, at 1,655,468 to 1,655,719
_07525
PS417_07530: PS417_07530 - multidrug transporter, at 1,655,733 to 1,657,127
_07530
PS417_07535: PS417_07535 - erythronate-4-phosphate dehydrogenase, at 1,657,268 to 1,658,410
_07535
PS417_07540: PS417_07540 - multifunctional fatty acid oxidation complex subunit alpha, at 1,658,464 to 1,658,637
_07540
Group
Condition
PS417
_07520
PS417
_07525
PS417
_07530
PS417
_07535
PS417
_07540
stress
Cisplatin 0.01 mg/ml
N.D.
N.D.
-1.9
N.D.
-0.1
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
N.D.
-0.0
N.D.
-1.5
motility
inner cut, LB soft agar motility assay
N.D.
N.D.
-0.3
N.D.
-0.9
motility
inner cut, LB soft agar motility assay
N.D.
N.D.
-0.2
N.D.
-0.9
phage
JP1 MOI 10
N.D.
N.D.
-0.4
N.D.
-0.7
phage
P. simiae ORA MOI 1
N.D.
N.D.
-0.1
N.D.
-0.8
in planta
Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
N.D.
N.D.
+0.3
N.D.
-1.2
supernatant
Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x
N.D.
N.D.
+0.1
N.D.
-0.9
stress
Phosphomycin 0.002 mg/ml
N.D.
N.D.
+0.1
N.D.
-0.9
solid stress
80% Methanol 2 mM; solid stress
N.D.
N.D.
+0.1
N.D.
-0.9
supernatant
RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x
N.D.
N.D.
+0.4
N.D.
-1.0
carbon source
octanoate (C)
N.D.
N.D.
+0.2
N.D.
-0.8
soil
soil sample 4; outgrowth in LB
N.D.
N.D.
+0.5
N.D.
-0.8
rhizosphere
rhizosphere sample 2; outgrowth in LB
N.D.
N.D.
+0.4
N.D.
-0.7
agar plate interaction control
Taped volatile agar plate with no fungus
N.D.
N.D.
+0.3
N.D.
-0.6
seeds
Growth on radish seeds for 72 hours
N.D.
N.D.
-0.5
N.D.
+0.4
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
N.D.
-0.4
N.D.
+0.7
rhizosphere
rhizosphere sample 1; outgrowth in LB
N.D.
N.D.
+0.8
N.D.
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
N.D.
N.D.
+0.1
N.D.
+0.8
stress
methylglyoxal 0.032 vol%
N.D.
N.D.
-0.3
N.D.
+1.3
phage
P. simiae ORA MOI 0.1
N.D.
N.D.
+0.2
N.D.
+0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
+0.3
N.D.
+0.7
carbon source
2'-Deoxyinosine 5 mM (C)
N.D.
N.D.
+0.4
N.D.
+0.5
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
N.D.
N.D.
+0.6
N.D.
+0.5
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
N.D.
N.D.
+0.3
N.D.
+0.9
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
+0.4
N.D.
+0.8
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
N.D.
-0.2
N.D.
+1.6
root
root sample 6; outgrowth in LB
N.D.
N.D.
+0.7
N.D.
+0.7
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
N.D.
+0.2
N.D.
+1.3
liquid stress
Fraxetin 2 mM; liquid stress
N.D.
N.D.
+0.3
N.D.
+1.2
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