Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_07335 and PS417_07340 are separated by 132 nucleotidesPS417_07340 and PS417_07345 are separated by 54 nucleotidesPS417_07345 and PS417_07350 are separated by 30 nucleotidesPS417_07350 and PS417_07355 are separated by 112 nucleotides PS417_07335: PS417_07335 - DSBA oxidoreductase, at 1,616,779 to 1,618,296 _07335 PS417_07340: PS417_07340 - calcium channel protein, at 1,618,429 to 1,619,268 _07340 PS417_07345: PS417_07345 - cyanate hydratase, at 1,619,323 to 1,619,793 _07345 PS417_07350: PS417_07350 - carbonic anhydrase, at 1,619,824 to 1,620,456 _07350 PS417_07355: PS417_07355 - transcriptional regulator, at 1,620,569 to 1,621,435 _07355
Group Condition PS417_07335 PS417_07340 PS417_07345 PS417_07350 PS417_07355
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 -0.1 N.D. N.D. -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.3 -0.0 N.D. N.D. -0.7
phage JP1 MOI 10 -0.3 -0.6 N.D. N.D. -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.4 -0.2 N.D. N.D. -0.3
soil soil sample 2; outgrowth in LB -0.0 -0.3 N.D. N.D. -0.6
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x -0.2 -0.5 N.D. N.D. -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 -0.1 N.D. N.D. -0.7
phage JP1 MOI 0.1 -0.4 -0.4 N.D. N.D. +0.2
phage P. simiae OR Antrim MOI 10 +0.2 -0.1 N.D. N.D. -0.6
phage P. simiae OR1 MOI 0.1 -0.3 -0.5 N.D. N.D. +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.2 +0.3 N.D. N.D. -0.4
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.5 -0.1 N.D. N.D. +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 -0.4 N.D. N.D. +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 -0.2 N.D. N.D. -0.4
nophagecontrol Only library +0.4 -0.4 N.D. N.D. -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.3 +0.5 N.D. N.D. -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 +0.2 N.D. N.D. -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.5 -0.1 N.D. N.D. -0.4
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.5 +0.1 N.D. N.D. +0.5
carbon source 2'-Deoxyinosine 5 mM (C) +0.4 +0.2 N.D. N.D. -0.5
phage P. simiae OR Antrim MOI 0.1 +0.4 +0.1 N.D. N.D. -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.4 +0.2 N.D. N.D. +0.5
soil soil sample 6; outgrowth in LB +0.6 +0.1 N.D. N.D. -0.3
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.6 +0.2 N.D. N.D. -0.3
carbon source L-Valine (C) +0.2 -0.2 N.D. N.D. +0.5
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.0 -0.1 N.D. N.D. -0.2
stress Gentamicin 0.008 mg/ml +0.3 -0.0 N.D. N.D. +0.5
agar plate interaction control Taped volatile agar plate with no fungus +0.3 +0.2 N.D. N.D. +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 +0.1 N.D. N.D. +0.4
stress Doxycycline hyclate 0.001 mg/ml +0.4 +0.3 N.D. N.D. +0.6
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