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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_07335 and PS417_07340 are separated by 132 nucleotides
PS417_07340 and PS417_07345 are separated by 54 nucleotides
PS417_07345 and PS417_07350 are separated by 30 nucleotides
PS417_07350 and PS417_07355 are separated by 112 nucleotides
PS417_07335: PS417_07335 - DSBA oxidoreductase, at 1,616,779 to 1,618,296
_07335
PS417_07340: PS417_07340 - calcium channel protein, at 1,618,429 to 1,619,268
_07340
PS417_07345: PS417_07345 - cyanate hydratase, at 1,619,323 to 1,619,793
_07345
PS417_07350: PS417_07350 - carbonic anhydrase, at 1,619,824 to 1,620,456
_07350
PS417_07355: PS417_07355 - transcriptional regulator, at 1,620,569 to 1,621,435
_07355
Group
Condition
PS417
_07335
PS417
_07340
PS417
_07345
PS417
_07350
PS417
_07355
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.5
-0.1
N.D.
N.D.
-0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.3
-0.0
N.D.
N.D.
-0.7
phage
JP1 MOI 10
-0.3
-0.6
N.D.
N.D.
-0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-0.4
-0.2
N.D.
N.D.
-0.3
soil
soil sample 2; outgrowth in LB
-0.0
-0.3
N.D.
N.D.
-0.6
supernatant
Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
-0.2
-0.5
N.D.
N.D.
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
-0.1
N.D.
N.D.
-0.7
phage
JP1 MOI 0.1
-0.4
-0.4
N.D.
N.D.
+0.2
phage
P. simiae OR Antrim MOI 10
+0.2
-0.1
N.D.
N.D.
-0.6
phage
P. simiae OR1 MOI 0.1
-0.3
-0.5
N.D.
N.D.
+0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.2
+0.3
N.D.
N.D.
-0.4
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.5
-0.1
N.D.
N.D.
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
-0.4
N.D.
N.D.
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
-0.2
N.D.
N.D.
-0.4
nophagecontrol
Only library
+0.4
-0.4
N.D.
N.D.
-0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.3
+0.5
N.D.
N.D.
-0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.2
+0.2
N.D.
N.D.
-0.4
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.5
-0.1
N.D.
N.D.
-0.4
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.5
+0.1
N.D.
N.D.
+0.5
carbon source
2'-Deoxyinosine 5 mM (C)
+0.4
+0.2
N.D.
N.D.
-0.5
phage
P. simiae OR Antrim MOI 0.1
+0.4
+0.1
N.D.
N.D.
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.4
+0.2
N.D.
N.D.
+0.5
soil
soil sample 6; outgrowth in LB
+0.6
+0.1
N.D.
N.D.
-0.3
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.6
+0.2
N.D.
N.D.
-0.3
carbon source
L-Valine (C)
+0.2
-0.2
N.D.
N.D.
+0.5
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+1.0
-0.1
N.D.
N.D.
-0.2
stress
Gentamicin 0.008 mg/ml
+0.3
-0.0
N.D.
N.D.
+0.5
agar plate interaction control
Taped volatile agar plate with no fungus
+0.3
+0.2
N.D.
N.D.
+0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.3
+0.1
N.D.
N.D.
+0.4
stress
Doxycycline hyclate 0.001 mg/ml
+0.4
+0.3
N.D.
N.D.
+0.6
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