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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_07305 and PS417_07310 are separated by 34 nucleotides
PS417_07310 and PS417_07315 are separated by 104 nucleotides
PS417_07315 and PS417_07325 are separated by 318 nucleotides
PS417_07325 and PS417_07330 are separated by 180 nucleotides
PS417_07305: PS417_07305 - 3-beta hydroxysteroid dehydrogenase, at 1,611,616 to 1,612,230
_07305
PS417_07310: PS417_07310 - beta-lactamase, at 1,612,265 to 1,613,131
_07310
PS417_07315: PS417_07315 - LysR family transcriptional regulator, at 1,613,236 to 1,614,141
_07315
PS417_07325: PS417_07325 - AraC family transcriptional regulator, at 1,614,460 to 1,615,338
_07325
PS417_07330: PS417_07330 - 4-hydroxybenzoate 3-monooxygenase, at 1,615,519 to 1,616,712
_07330
Group
Condition
PS417
_07305
PS417
_07310
PS417
_07315
PS417
_07325
PS417
_07330
agar plate interaction control
Taped volatile agar plate with no fungus
-1.3
-0.4
-0.8
+0.3
-0.9
seeds
Growth on radish seeds for 72 hours
-2.4
-0.2
-0.4
+0.3
+0.1
stress
methylglyoxal 0.032 vol%
-0.9
+0.3
-1.2
-0.4
+0.1
phage
P. simiae OR Antrim MOI 10
-1.9
-0.1
-0.2
+0.3
-0.1
seeds
Growth on radish seeds for 72 hours
-1.9
+0.5
-1.2
+0.3
+0.5
liquid stress
Fraxetin 3 mM; liquid stress
-1.1
-0.2
-0.3
+0.1
-0.4
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-1.4
+0.3
-0.5
-0.4
-0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.5
-0.2
-0.1
-0.1
+0.2
phage
P. simiae ORA MOI 10
-2.2
+0.4
-0.1
-0.0
+0.2
phage
JP1 MOI 0.1
-1.6
+0.2
-0.1
-0.4
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.3
-0.1
-0.5
+0.2
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.0
-0.3
+0.1
-0.6
+0.3
motility
inner cut, LB soft agar motility assay
-1.8
+0.2
+0.4
-0.0
-0.1
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.9
-0.6
+0.3
-0.2
+0.1
rhizosphere
rhizosphere sample 2; outgrowth in LB
-1.4
-0.1
-0.4
+0.8
-0.1
stress
TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 1
-1.5
+0.1
-0.3
+0.1
+0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.5
-0.1
-0.0
+0.5
-0.1
supernatant control
Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
-1.9
+0.3
+0.1
+0.2
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.3
+0.1
+0.2
-0.3
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.5
-0.1
+0.1
+0.2
+0.5
root
root sample 6; outgrowth in LB
-0.9
-0.2
+0.1
+0.6
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.0
-0.4
+0.2
+0.7
-0.2
rhizosphere
rhizosphere sample 1; outgrowth in LB
-0.7
-0.2
-0.5
+0.8
-0.0
seeds
Growth on radish seeds for 72 hours
-1.7
+0.8
+0.3
-0.0
+0.3
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.6
-0.5
-0.6
+0.7
-0.3
carbon source
L-Valine (C)
+0.7
+0.6
-0.5
+0.5
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+1.1
-0.1
-0.5
+0.5
+0.2
carbon source
2-Deoxy-D-Ribose 10 mM (C)
-0.4
-0.1
+1.2
+0.5
+0.1
in planta
Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+1.5
+0.1
+0.2
-0.1
-0.2
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+2.3
+0.0
-0.0
+0.1
-0.2
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