Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_07305 and PS417_07310 are separated by 34 nucleotidesPS417_07310 and PS417_07315 are separated by 104 nucleotidesPS417_07315 and PS417_07325 are separated by 318 nucleotidesPS417_07325 and PS417_07330 are separated by 180 nucleotides PS417_07305: PS417_07305 - 3-beta hydroxysteroid dehydrogenase, at 1,611,616 to 1,612,230 _07305 PS417_07310: PS417_07310 - beta-lactamase, at 1,612,265 to 1,613,131 _07310 PS417_07315: PS417_07315 - LysR family transcriptional regulator, at 1,613,236 to 1,614,141 _07315 PS417_07325: PS417_07325 - AraC family transcriptional regulator, at 1,614,460 to 1,615,338 _07325 PS417_07330: PS417_07330 - 4-hydroxybenzoate 3-monooxygenase, at 1,615,519 to 1,616,712 _07330
Group Condition PS417_07305 PS417_07310 PS417_07315 PS417_07325 PS417_07330
agar plate interaction control Taped volatile agar plate with no fungus -1.3 -0.4 -0.8 +0.3 -0.9
seeds Growth on radish seeds for 72 hours -2.4 -0.2 -0.4 +0.3 +0.1
stress methylglyoxal 0.032 vol% -0.9 +0.3 -1.2 -0.4 +0.1
phage P. simiae OR Antrim MOI 10 -1.9 -0.1 -0.2 +0.3 -0.1
seeds Growth on radish seeds for 72 hours -1.9 +0.5 -1.2 +0.3 +0.5
liquid stress Fraxetin 3 mM; liquid stress -1.1 -0.2 -0.3 +0.1 -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -1.4 +0.3 -0.5 -0.4 -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.5 -0.2 -0.1 -0.1 +0.2
phage P. simiae ORA MOI 10 -2.2 +0.4 -0.1 -0.0 +0.2
phage JP1 MOI 0.1 -1.6 +0.2 -0.1 -0.4 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.3 -0.1 -0.5 +0.2 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.0 -0.3 +0.1 -0.6 +0.3
motility inner cut, LB soft agar motility assay -1.8 +0.2 +0.4 -0.0 -0.1
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.9 -0.6 +0.3 -0.2 +0.1
rhizosphere rhizosphere sample 2; outgrowth in LB -1.4 -0.1 -0.4 +0.8 -0.1
stress TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 1 -1.5 +0.1 -0.3 +0.1 +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.5 -0.1 -0.0 +0.5 -0.1
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB -1.9 +0.3 +0.1 +0.2 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.3 +0.1 +0.2 -0.3 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.5 -0.1 +0.1 +0.2 +0.5
root root sample 6; outgrowth in LB -0.9 -0.2 +0.1 +0.6 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.0 -0.4 +0.2 +0.7 -0.2
rhizosphere rhizosphere sample 1; outgrowth in LB -0.7 -0.2 -0.5 +0.8 -0.0
seeds Growth on radish seeds for 72 hours -1.7 +0.8 +0.3 -0.0 +0.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.6 -0.5 -0.6 +0.7 -0.3
carbon source L-Valine (C) +0.7 +0.6 -0.5 +0.5 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +1.1 -0.1 -0.5 +0.5 +0.2
carbon source 2-Deoxy-D-Ribose 10 mM (C) -0.4 -0.1 +1.2 +0.5 +0.1
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.5 +0.1 +0.2 -0.1 -0.2
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +2.3 +0.0 -0.0 +0.1 -0.2
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