Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_07290 and PS417_07295 overlap by 4 nucleotidesPS417_07295 and PS417_07300 are separated by 111 nucleotidesPS417_07300 and PS417_07305 are separated by 55 nucleotidesPS417_07305 and PS417_07310 are separated by 34 nucleotides PS417_07290: PS417_07290 - aldehyde dehydrogenase, at 1,607,506 to 1,608,948 _07290 PS417_07295: PS417_07295 - hypothetical protein, at 1,608,945 to 1,610,570 _07295 PS417_07300: PS417_07300 - LysR family transcriptional regulator, at 1,610,682 to 1,611,560 _07300 PS417_07305: PS417_07305 - 3-beta hydroxysteroid dehydrogenase, at 1,611,616 to 1,612,230 _07305 PS417_07310: PS417_07310 - beta-lactamase, at 1,612,265 to 1,613,131 _07310
Group Condition PS417_07290 PS417_07295 PS417_07300 PS417_07305 PS417_07310
seeds Growth on radish seeds for 72 hours +0.4 -0.3 -0.5 -2.4 -0.2
agar plate interaction control Taped volatile agar plate with no fungus -0.4 -0.1 -0.6 -1.3 -0.4
phage P. simiae OR Antrim MOI 10 +0.0 +0.4 -0.8 -1.9 -0.1
rhizosphere rhizosphere sample 2; outgrowth in LB -0.2 -0.2 -0.4 -1.4 -0.1
phage P. simiae ORA MOI 10 -0.1 -0.2 -0.2 -2.2 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.0 -0.4 -0.7 -1.0 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 -0.2 -0.4 -1.5 -0.1
solid stress 80% Methanol 0.5 mM; solid stress +0.1 -0.5 +0.0 -1.3 -0.3
seeds Growth on radish seeds for 72 hours +0.0 -0.4 -0.1 -1.9 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 -0.1 -0.2 -1.5 -0.2
motility inner cut, LB soft agar motility assay +0.1 -0.2 -0.0 -1.8 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.3 +0.3 -0.4 -1.2 -0.1
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.1 -0.7 -0.5 -0.9 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 +0.3 -0.6 -1.0 -0.4
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB +0.1 -0.4 +0.4 -1.9 +0.3
seeds Growth on radish seeds for 72 hours +0.2 -0.6 -0.2 -1.7 +0.8
phage P. simiae Grant Run MOI 10 +0.4 -0.2 -0.5 -0.9 -0.1
stress methylglyoxal 0.032 vol% +0.3 -0.7 -0.1 -0.9 +0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.0 +0.7 -0.2 -1.5 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.1 +0.4 +0.2 -1.7 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.2 -0.1 +0.5 -1.4 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.4 +0.8 -0.2 -1.4 +0.3
carbon source 2'-Deoxyinosine 5 mM (C) -0.4 -0.4 -0.8 +0.9 -0.3
phage JP1 MOI 0.1 +0.3 +0.5 -0.1 -1.6 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 +0.2 +0.3 -1.3 +0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 +0.1 +0.9 -0.6 +0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -0.3 +0.7 +0.7 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 +0.1 +0.7 +1.1 -0.1
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.1 +0.3 -0.4 +1.5 +0.1
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 +0.1 -0.0 +2.3 +0.0
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