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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_07280 and PS417_07285 are separated by 137 nucleotides
PS417_07285 and PS417_07290 are separated by 213 nucleotides
PS417_07290 and PS417_07295 overlap by 4 nucleotides
PS417_07295 and PS417_07300 are separated by 111 nucleotides
PS417_07280: PS417_07280 - LysR family transcriptional regulator, at 1,605,032 to 1,605,967
_07280
PS417_07285: PS417_07285 - MFS transporter, at 1,606,105 to 1,607,292
_07285
PS417_07290: PS417_07290 - aldehyde dehydrogenase, at 1,607,506 to 1,608,948
_07290
PS417_07295: PS417_07295 - hypothetical protein, at 1,608,945 to 1,610,570
_07295
PS417_07300: PS417_07300 - LysR family transcriptional regulator, at 1,610,682 to 1,611,560
_07300
Group
Condition
PS417
_07280
PS417
_07285
PS417
_07290
PS417
_07295
PS417
_07300
carbon source
2'-Deoxyinosine 5 mM (C)
-0.0
-0.3
-0.4
-0.4
-0.8
rhizosphere
rhizosphere sample 2; outgrowth in LB
-0.8
-0.1
-0.2
-0.2
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.5
+0.1
-0.4
-0.2
-0.8
phage
P. simiae ORA MOI 0.1
-0.1
-0.4
+0.1
+0.0
-0.9
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
-0.3
+0.2
-0.1
-0.6
phage
JP1 MOI 1
-0.9
+0.1
-0.1
-0.5
+0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
-0.4
-0.4
+0.1
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Threonine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
-0.4
-0.1
-0.7
+0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.4
-0.4
-0.7
-0.0
+0.1
phage
JP1 MOI 10
-0.6
-0.1
-0.3
-0.2
+0.7
phage
P. simiae Grant Run MOI 10
+0.1
-0.4
+0.4
-0.2
-0.5
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.2
+0.4
-0.4
+0.1
-0.9
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
+0.2
+0.1
-0.1
-0.9
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.4
-0.0
+0.2
+0.3
-0.6
motility
outer cut, LB soft agar motility assay
-0.2
-0.4
-0.2
-0.2
+0.5
phage
P. simiae Grant Run MOI 0.1
-0.5
-0.1
+0.4
+0.1
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.4
-0.2
-0.5
+0.1
+0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.1
+0.1
-0.4
-0.5
+0.5
carbon source
2-Deoxy-D-ribonic lithium salt 10 mM (C)
+0.6
+0.1
-0.2
-0.0
-0.6
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.1
+0.1
+1.1
-0.7
-0.5
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.2
-0.2
+0.2
+0.4
-0.7
seeds
Growth on radish seeds for 72 hours
+0.6
-0.2
+0.4
-0.3
-0.5
seeds
Growth on radish seeds for 24 hours
+0.9
-0.7
+0.4
+0.1
-0.7
phage
P. simiae OR Antrim MOI 10
+0.3
+0.2
+0.0
+0.4
-0.8
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.3
+0.6
+0.0
+0.5
-0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.4
+0.1
-0.4
+0.8
-0.2
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.3
+0.4
+0.7
+0.1
-0.5
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.1
+0.2
+0.2
+0.1
+0.9
seeds
Growth on radish seeds for 72 hours
+1.7
+0.2
+0.2
-0.6
-0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.9
+0.1
+0.2
-0.3
+0.7
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