Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_07025 and PS417_07030 are separated by 52 nucleotidesPS417_07030 and PS417_07035 are separated by 136 nucleotidesPS417_07035 and PS417_07040 are separated by 212 nucleotidesPS417_07040 and PS417_07045 are separated by 193 nucleotides PS417_07025: PS417_07025 - hypothetical protein, at 1,549,486 to 1,549,851 _07025 PS417_07030: PS417_07030 - enoyl-CoA hydratase, at 1,549,904 to 1,551,016 _07030 PS417_07035: PS417_07035 - uracil-DNA glycosylase, at 1,551,153 to 1,551,845 _07035 PS417_07040: PS417_07040 - hypothetical protein, at 1,552,058 to 1,554,079 _07040 PS417_07045: PS417_07045 - hypothetical protein, at 1,554,273 to 1,556,234 _07045
Group Condition PS417_07025 PS417_07030 PS417_07035 PS417_07040 PS417_07045
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.1 -0.3 -1.7 +0.1 +0.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.2 -0.1 -1.5 +0.2 +0.0
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.0 +0.1 -1.6 +0.0 -0.0
r2a control with 0.2x vogels R2A control with 0.2X Vogels -0.2 +0.0 -1.5 +0.1 +0.0
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.2 +0.0 -1.2 -0.1 -0.1
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.1 -0.3 -1.3 +0.1 +0.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.1 -0.2 -1.3 +0.2 -0.1
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.1 -0.3 -1.0 +0.0 -0.0
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.2 +0.1 -1.3 -0.0 -0.1
r2a control with 0.2x vogels R2A control with 0.2X Vogels -0.1 +0.1 -1.3 +0.1 -0.0
phage P. simiae OR1 MOI 0.1 +0.1 -0.3 -0.8 -0.1 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.1 -0.2 -0.8 +0.2 -0.2
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X -0.0 -0.1 -0.9 +0.1 -0.0
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.4 -0.2 -1.3 +0.1 -0.0
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.1 -0.3 -1.0 +0.2 +0.1
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.1 -0.1 -1.0 +0.1 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.1 -0.6 -0.5 +0.1 +0.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.1 -0.1 -1.0 +0.2 +0.1
phage P. simiae ORA MOI 0.1 -0.0 +0.2 -1.1 +0.1 +0.1
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 -0.2 -0.5 +0.0 -0.1
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -0.1 +0.4 -0.7 +0.0 +0.1
rhizosphere rhizosphere sample 1; outgrowth in LB -0.1 -0.5 +0.6 -0.2 +0.1
carbon source 2'-Deoxyinosine 5 mM (C) -0.4 +0.5 -0.0 +0.1 -0.2
nophagecontrol Only library -0.6 +0.4 +0.1 +0.1 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 +0.4 +0.5 +0.0 -0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.1 -0.3 +0.8 +0.0 +0.2
solid stress Fraxetin 3 mM; solid stress +0.4 +0.3 +0.4 +0.0 -0.1
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 +0.1 +0.5 -0.1 +0.4
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.1 +1.1 +0.1 +0.0 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.0 +0.6 +0.6 +0.2 -0.2
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