Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_00675 and PS417_00680 are separated by 92 nucleotidesPS417_00680 and PS417_00685 are separated by 130 nucleotidesPS417_00685 and PS417_00690 overlap by 4 nucleotidesPS417_00690 and PS417_00695 are separated by 168 nucleotides PS417_00675: PS417_00675 - iron dicitrate transport regulator FecR, at 138,327 to 139,277 _00675 PS417_00680: PS417_00680 - TonB-dependent receptor, at 139,370 to 141,823 _00680 PS417_00685: PS417_00685 - hypothetical protein, at 141,954 to 142,658 _00685 PS417_00690: PS417_00690 - hypothetical protein, at 142,655 to 144,823 _00690 PS417_00695: PS417_00695 - membrane protein, at 144,992 to 146,596 _00695
Group Condition PS417_00675 PS417_00680 PS417_00685 PS417_00690 PS417_00695
carbon source 2'-Deoxyinosine 5 mM (C) -0.9 +0.1 -0.4 -0.0 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 -0.2 -0.7 -0.2 -0.1
carbon source 2-Deoxy-D-ribonic lithium salt 10 mM (C) -0.5 +0.0 -0.4 +0.0 -0.3
nophagecontrol Only library -0.5 +0.3 -1.1 +0.2 +0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 +0.1 -1.0 +0.0 -0.2
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.3 +0.3 -0.8 +0.2 -0.3
agar plate interaction control Taped volatile agar plate with no fungus -0.5 +0.2 -0.6 -0.1 -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.7 +0.2 -0.1 -0.1 -0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.0 +0.0 +0.2 +0.1 -0.1
soil soil sample 3; outgrowth in LB +0.2 -0.0 -1.1 -0.0 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.6 -0.2 +0.1 -0.3 +0.3
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.7 -0.1 +0.3 -0.1 -0.1
solid stress Fraxetin 3 mM; solid stress -0.7 -0.0 +0.3 -0.1 +0.2
rhizosphere rhizosphere sample 2; outgrowth in LB -0.6 +0.1 +0.4 -0.2 +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.5 +0.1 +0.5 -0.0 -0.2
phage JP1 MOI 1 +0.4 +0.2 -0.8 +0.4 -0.0
phage P. simiae ORA MOI 0.1 -0.6 +0.2 +0.2 +0.2 +0.2
soil soil sample 8; outgrowth in LB -0.5 +0.2 +0.6 +0.0 -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.6 -0.2 +0.8 -0.1 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 -0.1 +0.8 -0.3 -0.0
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 -0.3 +0.4 -0.2 -0.1
stress Gentamicin 0.008 mg/ml -0.4 -0.1 +0.5 +0.3 +0.1
phage P. simiae Grant Run MOI 0.1 +0.5 +0.1 +0.3 -0.2 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +1.2 +0.0 -0.3 -0.1 +0.0
phage JP1 MOI 10 +0.5 -0.1 +0.2 +0.4 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 -0.0 +0.7 +0.3 +0.1
soil soil sample 4; outgrowth in LB +0.2 +0.0 +0.9 +0.2 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 +0.1 +0.7 +0.1 +0.0
phage P. simiae OR1 MOI 0.1 +0.2 +0.1 +0.4 +0.7 +0.1
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +1.2 -0.0 +1.6 +0.1 -0.1
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