Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_06575 and PS417_06580 overlap by 4 nucleotidesPS417_06580 and PS417_06585 overlap by 4 nucleotidesPS417_06585 and PS417_06590 are separated by 23 nucleotidesPS417_06590 and PS417_06595 are separated by 260 nucleotides PS417_06575: PS417_06575 - DoxX, at 1,457,129 to 1,457,614 _06575 PS417_06580: PS417_06580 - hypothetical protein, at 1,457,611 to 1,458,387 _06580 PS417_06585: PS417_06585 - hypothetical protein, at 1,458,384 to 1,459,262 _06585 PS417_06590: PS417_06590 - membrane protein, at 1,459,286 to 1,459,582 _06590 PS417_06595: PS417_06595 - ABC transporter ATP-binding protein, at 1,459,843 to 1,460,544 _06595
Group Condition PS417_06575 PS417_06580 PS417_06585 PS417_06590 PS417_06595
phage P. simiae ORA MOI 10 -0.4 -0.7 -0.1 N.D. N.D.
root root sample 6; outgrowth in LB -0.7 -0.2 -0.1 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 -0.4 -0.1 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 -0.5 -0.2 N.D. N.D.
solid stress Fraxetin 0.5 mM; solid stress -0.4 -0.2 -0.4 N.D. N.D.
soil soil sample 6; outgrowth in LB -0.6 -0.1 -0.3 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 -0.8 +0.3 N.D. N.D.
supernatant control Vogels_fungal_media 0.1X -0.5 +0.2 -0.4 N.D. N.D.
stress methylglyoxal 0.032 vol% -0.0 -0.9 +0.3 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -0.1 -0.7 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -0.9 +0.2 N.D. N.D.
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x +0.3 -0.3 -0.3 N.D. N.D.
carbon source 2-Deoxy-D-ribonic lithium salt 10 mM (C) +0.4 -0.3 -0.3 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 -0.4 -0.2 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.2 +0.4 -0.2 N.D. N.D.
phage P. simiae OR Antrim MOI 10 -0.4 +0.5 +0.1 N.D. N.D.
agar plate interaction control Taped volatile agar plate with no fungus +0.6 -0.2 -0.2 N.D. N.D.
phage Wcs_1 +0.5 -0.4 +0.3 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.6 +0.0 -0.3 N.D. N.D.
supernatant LB 0.5x with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x +0.5 +0.2 -0.3 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.5 +0.2 -0.2 N.D. N.D.
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.1 -0.2 +0.6 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.7 -0.2 +0.2 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.9 -0.1 -0.1 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.7 +0.0 +0.2 N.D. N.D.
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB +0.4 +0.3 +0.3 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.2 -0.2 -0.0 N.D. N.D.
carbon source L-Valine (C) +0.8 -0.0 +0.3 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Aspartic Acid; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.5 +0.6 +0.1 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.6 -0.1 -0.2 N.D. N.D.
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