Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_06485 and PS417_06490 are separated by 4 nucleotidesPS417_06490 and PS417_06495 are separated by 190 nucleotidesPS417_06495 and PS417_06500 are separated by 160 nucleotidesPS417_06500 and PS417_06505 are separated by 119 nucleotides PS417_06485: PS417_06485 - hypothetical protein, at 1,437,843 to 1,438,313 _06485 PS417_06490: PS417_06490 - hypothetical protein, at 1,438,318 to 1,438,815 _06490 PS417_06495: PS417_06495 - membrane protein, at 1,439,006 to 1,439,701 _06495 PS417_06500: PS417_06500 - hypothetical protein, at 1,439,862 to 1,440,620 _06500 PS417_06505: PS417_06505 - membrane protein, at 1,440,740 to 1,441,204 _06505
Group Condition PS417_06485 PS417_06490 PS417_06495 PS417_06500 PS417_06505
liquid stress Scopoletin 3 mM; liquid stress -0.1 -0.1 N.D. -2.6 -0.2
rhizosphere rhizosphere sample 2; outgrowth in LB -0.4 +0.4 N.D. -3.1 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.0 -0.6 N.D. -1.6 -0.1
root root sample 6; outgrowth in LB +0.1 -0.5 N.D. -1.7 +0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.0 +0.5 N.D. -2.3 -0.1
carbon source L-Valine (C) -0.0 +0.1 N.D. -2.1 +0.2
soil soil sample 4; outgrowth in LB +0.1 +0.1 N.D. -2.6 +0.6
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 -0.5 N.D. -1.5 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 +0.4 N.D. -1.1 -0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 -1.0 N.D. -0.9 +0.5
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.1 +0.2 N.D. -2.1 +0.8
motility inner cut, LB soft agar motility assay +0.2 +0.5 N.D. -1.6 +0.0
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -0.5 -1.4 N.D. +1.0 +0.1
rhizosphere rhizosphere sample 8; outgrowth in LB +0.2 +0.6 N.D. -1.4 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 -1.3 N.D. +0.8 -0.2
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x +0.4 +0.6 N.D. -1.5 -0.0
seeds Growth on radish seeds for 72 hours +0.7 -1.2 N.D. -0.1 +0.4
phage P. simiae OR1 MOI 0.1 -0.3 +0.9 N.D. -1.2 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.8 +0.3 N.D. +1.0 -0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.5 -0.2 N.D. +1.1 -0.5
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 -0.3 N.D. +1.2 -0.5
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.6 -0.2 N.D. -1.0 +0.7
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x +0.2 +0.3 N.D. -1.1 +1.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 -0.3 N.D. +1.5 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.8 -0.9 N.D. +0.7 +0.1
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.2 -0.4 N.D. +1.4 +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 -1.3 N.D. +1.6 +0.8
soil soil sample 2; outgrowth in LB -0.1 +0.5 N.D. +1.4 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 -0.5 N.D. +1.7 +0.2
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.3 +0.4 N.D. +1.5 +0.1
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