Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_06475 and PS417_06480 overlap by 4 nucleotidesPS417_06480 and PS417_06485 are separated by 20 nucleotidesPS417_06485 and PS417_06490 are separated by 4 nucleotidesPS417_06490 and PS417_06495 are separated by 190 nucleotides PS417_06475: PS417_06475 - zinc chelation protein SecC, at 1,437,143 to 1,437,343 _06475 PS417_06480: PS417_06480 - hypothetical protein, at 1,437,340 to 1,437,822 _06480 PS417_06485: PS417_06485 - hypothetical protein, at 1,437,843 to 1,438,313 _06485 PS417_06490: PS417_06490 - hypothetical protein, at 1,438,318 to 1,438,815 _06490 PS417_06495: PS417_06495 - membrane protein, at 1,439,006 to 1,439,701 _06495
Group Condition PS417_06475 PS417_06480 PS417_06485 PS417_06490 PS417_06495
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.6 -0.5 -0.9 N.D.
phage P. simiae ORA MOI 0.1 N.D. -0.6 -0.2 -1.3 N.D.
motility inner cut, LB soft agar motility assay N.D. -1.3 +0.3 -0.8 N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X N.D. +0.1 -0.5 -1.4 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.5 +0.1 -1.3 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -1.0 -0.2 -0.5 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.3 -0.7 -0.7 N.D.
root root sample 6; outgrowth in LB N.D. -1.1 +0.1 -0.5 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.7 -0.0 -0.6 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.3 -0.7 -0.7 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.1 +0.2 -1.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -0.3 +0.2 -0.8 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -0.2 +0.3 -1.0 N.D.
seeds Growth on radish seeds for 72 hours N.D. -0.3 +0.7 -1.2 N.D.
soil soil sample 5; outgrowth in LB N.D. -1.0 -0.1 +0.5 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB N.D. +0.3 +0.0 -0.9 N.D.
seeds Growth on radish seeds for 72 hours N.D. -0.1 +0.4 -0.8 N.D.
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB N.D. +0.3 +0.3 -0.9 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days N.D. +0.1 +0.5 -0.8 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.5 +0.8 -0.9 N.D.
phage Wcs_1 N.D. -0.4 +0.0 +0.8 N.D.
phage P. simiae OR Antrim MOI 10 N.D. +0.6 -0.5 +0.3 N.D.
soil soil sample 6; outgrowth in LB N.D. -0.3 +0.5 +0.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.8 +0.4 -0.5 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. -0.5 +0.5 +0.8 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB N.D. +0.7 +0.3 -0.2 N.D.
carbon source 2'-Deoxyinosine 5 mM (C) N.D. +0.7 +0.3 -0.2 N.D.
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. +0.4 -0.1 +0.9 N.D.
agar plate interaction control Taped volatile agar plate with no fungus N.D. +0.7 +0.4 +0.3 N.D.
stress methylglyoxal 0.032 vol% N.D. +0.6 +0.2 +0.6 N.D.
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