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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_06450 and PS417_06455 are separated by 28 nucleotides
PS417_06455 and PS417_06460 are separated by 3 nucleotides
PS417_06460 and PS417_06465 are separated by 198 nucleotides
PS417_06465 and PS417_06470 overlap by 4 nucleotides
PS417_06450: PS417_06450 - iron ABC transporter substrate-binding protein, at 1,430,511 to 1,431,626
_06450
PS417_06455: PS417_06455 - Fe(II)-dependent oxygenase, at 1,431,655 to 1,432,335
_06455
PS417_06460: PS417_06460 - TonB-dependent receptor, at 1,432,339 to 1,434,720
_06460
PS417_06465: PS417_06465 - transcriptional regulator, at 1,434,919 to 1,435,629
_06465
PS417_06470: PS417_06470 - histidine kinase, at 1,435,626 to 1,437,011
_06470
Group
Condition
PS417
_06450
PS417
_06455
PS417
_06460
PS417
_06465
PS417
_06470
motility
inner cut, LB soft agar motility assay
+0.1
-0.5
-0.4
-1.1
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
-2.8
+0.1
+0.1
+0.1
phage
P. simiae OR Antrim MOI 1
-0.2
-1.2
-0.0
-0.5
-0.2
agar plate interaction control
Taped volatile agar plate with no fungus
+0.3
-0.8
-0.2
-0.3
-0.7
soil
soil sample 4; outgrowth in LB
+0.1
-0.9
+0.5
-0.9
-0.4
carbon source
L-Valine (C)
+0.0
-0.4
-0.1
-1.1
+0.0
seeds
Growth on radish seeds for 72 hours
-0.2
-1.0
+0.0
+0.1
-0.4
solid stress
Fraxetin 3 mM; solid stress
+0.1
-1.2
+0.1
-0.1
-0.3
phage
P. simiae ORA MOI 1
+0.0
-1.2
+0.2
+0.1
-0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
+0.1
-0.4
+0.3
-1.1
-0.1
rhizosphere
rhizosphere sample 2; outgrowth in LB
-0.2
-0.2
+0.5
-0.9
-0.0
soil
soil sample 3; outgrowth in LB
+0.1
-1.1
+0.5
+0.1
-0.3
soil
soil sample 5; outgrowth in LB
+0.0
-0.2
+0.5
-0.9
+0.0
in planta
Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.1
-0.6
+0.0
-0.7
+0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
-0.2
-0.8
+0.4
+0.2
nophagecontrol
Only library
+0.0
-0.7
-0.4
+0.2
+0.5
phage
P. simiae OR1 MOI 0.1
-0.0
-0.6
+0.5
-0.5
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
-0.4
-0.2
-0.3
+0.8
rhizosphere
rhizosphere sample 1; outgrowth in LB
+0.3
+0.3
+0.5
-0.7
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
-0.5
-0.3
+0.5
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.1
+0.5
+0.1
-0.7
+0.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
-0.5
+0.1
-0.2
+0.8
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.0
+0.6
+0.4
-0.6
+0.1
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.1
+1.0
-0.2
-0.3
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.2
+0.4
-0.1
+0.9
-0.1
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.1
+1.5
-0.1
-0.0
+0.3
phage
P. simiae OR Antrim MOI 10
-0.1
+0.7
+0.1
+1.1
-0.0
seeds
Growth on radish seeds for 72 hours
+0.0
+1.5
+0.1
-0.3
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.2
+0.4
-0.3
+1.5
+0.3
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.2
+0.1
-0.1
+1.6
+0.1
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