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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_06425 and PS417_06430 are separated by 122 nucleotides
PS417_06430 and PS417_06435 are separated by 102 nucleotides
PS417_06435 and PS417_06440 are separated by 105 nucleotides
PS417_06440 and PS417_06445 overlap by 4 nucleotides
PS417_06425: PS417_06425 - exonuclease, at 1,424,155 to 1,425,159
_06425
PS417_06430: PS417_06430 - acyl-homoserine lactone acylase subunit beta, at 1,425,282 to 1,427,723
_06430
PS417_06435: PS417_06435 - glutathione S-transferase, at 1,427,826 to 1,428,470
_06435
PS417_06440: PS417_06440 - iron ABC transporter, at 1,428,576 to 1,429,361
_06440
PS417_06445: PS417_06445 - iron-siderophore ABC transporter permease, at 1,429,358 to 1,430,416
_06445
Group
Condition
PS417
_06425
PS417
_06430
PS417
_06435
PS417
_06440
PS417
_06445
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.0
+0.4
-0.9
-0.1
-2.6
seeds
Growth on radish seeds for 72 hours
+0.3
-0.6
-0.3
+0.1
-1.9
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.5
-0.2
-0.2
-0.5
-0.8
carbon source
Carbon source D-Mannitol 2.5 mM
-0.1
+0.0
-0.1
-0.1
-1.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.7
+0.0
+0.0
+0.1
-1.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.3
+0.2
+0.0
+0.1
-1.7
phage
FRSphage
+0.0
-0.1
+0.3
-0.0
-1.7
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.1
+0.3
-0.5
-0.6
-0.6
carbon source
L-Leucine (C)
-0.2
-0.1
+0.5
+0.1
-1.6
liquid stress
80% Methanol 2 mM; liquid stress
-0.1
-0.1
+0.4
+0.1
-1.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.1
+0.1
-0.1
+0.6
-1.6
stress
Tetracycline 0.001 mg/ml
+0.2
-0.2
+0.4
+0.3
-1.6
phage
P. simiae OR1 MOI 0.1
-0.6
+0.2
-0.1
+0.4
-0.7
phage
P. simiae OR Antrim MOI 10
-0.5
+0.3
+0.5
-0.2
-0.9
phage
P. simiae OR Antrim MOI 1
-0.0
+0.2
+0.0
+0.7
-1.5
phage
P. simiae Grant Run MOI 0.1
+0.2
-0.1
+0.6
-0.3
-0.9
stress
methylglyoxal 0.032 vol%
+0.5
-0.0
+0.3
+0.3
-1.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.4
-0.3
+0.6
+0.1
-0.8
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.1
+0.2
+0.4
+0.4
-0.8
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
+0.1
+0.4
+0.6
-0.8
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.6
+0.5
-0.3
+0.1
-0.4
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.1
-0.0
-0.6
+0.6
+0.7
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.4
-0.4
-0.2
+0.2
+0.8
carbon source
L-Valine (C)
+0.1
-0.2
+0.5
-0.4
+1.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.4
-0.1
+0.7
-0.1
+1.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
-0.2
-0.6
+0.5
+1.9
supernatant
Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
+0.2
+0.3
+0.2
+0.3
+1.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.0
+0.2
+0.9
+0.9
+0.1
root
root sample 6; outgrowth in LB
+0.1
-0.0
+0.6
+0.4
+1.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
+0.5
+0.6
+0.6
+1.9
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