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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_06420 and PS417_06425 overlap by 4 nucleotides
PS417_06425 and PS417_06430 are separated by 122 nucleotides
PS417_06430 and PS417_06435 are separated by 102 nucleotides
PS417_06435 and PS417_06440 are separated by 105 nucleotides
PS417_06420: PS417_06420 - ATP-dependent DNA ligase, at 1,422,524 to 1,424,158
_06420
PS417_06425: PS417_06425 - exonuclease, at 1,424,155 to 1,425,159
_06425
PS417_06430: PS417_06430 - acyl-homoserine lactone acylase subunit beta, at 1,425,282 to 1,427,723
_06430
PS417_06435: PS417_06435 - glutathione S-transferase, at 1,427,826 to 1,428,470
_06435
PS417_06440: PS417_06440 - iron ABC transporter, at 1,428,576 to 1,429,361
_06440
Group
Condition
PS417
_06420
PS417
_06425
PS417
_06430
PS417
_06435
PS417
_06440
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+0.1
-0.5
-0.2
-0.2
-0.5
phage
P. simiae OR1 MOI 0.1
-0.8
-0.6
+0.2
-0.1
+0.4
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.2
+0.1
-0.2
-0.4
-0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
+0.3
-0.1
+0.3
-0.5
-0.6
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
+0.3
-0.0
+0.1
-0.8
-0.4
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.4
+0.4
-0.2
-0.2
-0.3
supernatant control
Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
+0.3
+0.0
+0.2
-0.6
-0.5
carbon source
2'-Deoxyinosine 5 mM (C)
-0.3
-0.6
-0.2
+0.4
-0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
-0.0
+0.4
-0.9
-0.1
agar plate interaction control
Taped volatile agar plate with no fungus
-0.2
-0.3
+0.5
-0.6
+0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
+0.1
-0.2
-0.6
+0.5
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.0
+0.1
-0.0
-0.6
+0.6
phage
P. simiae Grant Run MOI 0.1
-0.4
+0.2
-0.1
+0.6
-0.3
seeds
Growth on radish seeds for 72 hours
+0.5
+0.3
-0.6
-0.3
+0.1
seeds
Growth on radish seeds for 72 hours
-0.2
+0.5
+0.2
-0.7
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
+0.1
-0.2
-0.4
+0.3
phage
P. simiae OR Antrim MOI 10
+0.0
-0.5
+0.3
+0.5
-0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
+0.4
-0.1
+0.8
-0.4
-0.4
stress
Doxycycline hyclate 0.001 mg/ml
+0.4
+0.3
-0.4
+0.2
-0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.0
+0.2
+0.4
-0.7
+0.5
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.4
+0.6
+0.5
-0.3
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
+0.2
+0.0
-0.4
+0.3
seeds
Growth on radish seeds for 24 hours
+0.9
+0.2
+0.1
-0.1
-0.2
in planta
Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.3
+0.4
+0.3
+0.4
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.4
+0.4
-0.3
+0.6
+0.1
root
root sample 6; outgrowth in LB
+0.3
+0.1
-0.0
+0.6
+0.4
phage
P. simiae Grant Run MOI 10
-0.1
+0.3
+0.2
+0.4
+0.5
supernatant
Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
+0.4
+0.2
+0.3
+0.2
+0.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.2
-0.0
+0.2
+0.9
+0.9
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
+0.3
+0.5
+0.6
+0.6
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