Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_06420 and PS417_06425 overlap by 4 nucleotidesPS417_06425 and PS417_06430 are separated by 122 nucleotidesPS417_06430 and PS417_06435 are separated by 102 nucleotidesPS417_06435 and PS417_06440 are separated by 105 nucleotides PS417_06420: PS417_06420 - ATP-dependent DNA ligase, at 1,422,524 to 1,424,158 _06420 PS417_06425: PS417_06425 - exonuclease, at 1,424,155 to 1,425,159 _06425 PS417_06430: PS417_06430 - acyl-homoserine lactone acylase subunit beta, at 1,425,282 to 1,427,723 _06430 PS417_06435: PS417_06435 - glutathione S-transferase, at 1,427,826 to 1,428,470 _06435 PS417_06440: PS417_06440 - iron ABC transporter, at 1,428,576 to 1,429,361 _06440
Group Condition PS417_06420 PS417_06425 PS417_06430 PS417_06435 PS417_06440
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.1 -0.5 -0.2 -0.2 -0.5
phage P. simiae OR1 MOI 0.1 -0.8 -0.6 +0.2 -0.1 +0.4
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.2 +0.1 -0.2 -0.4 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.3 -0.1 +0.3 -0.5 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.3 -0.0 +0.1 -0.8 -0.4
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.4 +0.4 -0.2 -0.2 -0.3
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB +0.3 +0.0 +0.2 -0.6 -0.5
carbon source 2'-Deoxyinosine 5 mM (C) -0.3 -0.6 -0.2 +0.4 -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 -0.0 +0.4 -0.9 -0.1
agar plate interaction control Taped volatile agar plate with no fungus -0.2 -0.3 +0.5 -0.6 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 +0.1 -0.2 -0.6 +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.0 +0.1 -0.0 -0.6 +0.6
phage P. simiae Grant Run MOI 0.1 -0.4 +0.2 -0.1 +0.6 -0.3
seeds Growth on radish seeds for 72 hours +0.5 +0.3 -0.6 -0.3 +0.1
seeds Growth on radish seeds for 72 hours -0.2 +0.5 +0.2 -0.7 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.5 +0.1 -0.2 -0.4 +0.3
phage P. simiae OR Antrim MOI 10 +0.0 -0.5 +0.3 +0.5 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.4 -0.1 +0.8 -0.4 -0.4
stress Doxycycline hyclate 0.001 mg/ml +0.4 +0.3 -0.4 +0.2 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.0 +0.2 +0.4 -0.7 +0.5
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.4 +0.6 +0.5 -0.3 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.5 +0.2 +0.0 -0.4 +0.3
seeds Growth on radish seeds for 24 hours +0.9 +0.2 +0.1 -0.1 -0.2
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.3 +0.4 +0.3 +0.4 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.4 +0.4 -0.3 +0.6 +0.1
root root sample 6; outgrowth in LB +0.3 +0.1 -0.0 +0.6 +0.4
phage P. simiae Grant Run MOI 10 -0.1 +0.3 +0.2 +0.4 +0.5
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x +0.4 +0.2 +0.3 +0.2 +0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 -0.0 +0.2 +0.9 +0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 +0.3 +0.5 +0.6 +0.6
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